Combination of PI3K-inhibitors

ABSTRACT

The present invention relates to combinations of at least two components, component A and component B, component A being an inhibitor of PI3K kinase, and component B being radium 223, particularly a pharmaceutically acceptable salt of radium-223. Another aspect of the present invention relates to the use of such combinations as described herein for the preparation of a medicament for the treatment or prophylaxis of a disease, particularly for the treatment of breast and prostate cancer as well as their bone metatases.

CROSS REFERENCE TO RELATED APPLICATIONS

This application is a National Phase application under 35 U.S.C. § 371of International Application No. PCT/EP2014/075886, filed Nov. 28, 2014,which claims priority benefit of European Application No. 13195485.1,filed Dec. 3, 2013.

The present invention relates to combinations of at least twocomponents, component A and component B, component A being aPI3K-inhibitor, and component B being a pharmaceutically acceptable saltof the alkaline-earth radionuclide radium-223.

Another aspect of the present invention relates to the use of suchcombinations as described herein for the preparation of a medicament forthe treatment or prophylaxis of a disease, particularly for thetreatment of cancer.

Yet another aspect of the present invention relates to methods oftreatment or prophylaxis of a cancer in a subject, comprisingadministering to said subject a therapeutically effective amount of acombination as described herein.

Further, the present invention relates to a kit comprising a combinationof:

-   -   one or more components A, as defined herein, or a        physiologically acceptable salt, solvate, hydrate or        stereoisomer thereof;    -   a component B, as defined supra, or a solvate or hydrate        thereof; and, optionally    -   one or more pharmaceutical agents C;        in which optionally either or both of said components A and B        are in the form of a pharmaceutical formulation which is ready        for use to be administered simultaneously, concurrently,        separately or sequentially.

Component A may be administered by the oral, intravenous, topical, localinstallations, intraperitoneal or nasal route.

Component B preferably is administered by the intravenous route.

BACKGROUND TO THE INVENTION

Cancer is the second most prevalent cause of death in the United States,causing 450,000 deaths per year. While substantial progress has beenmade in identifying some of the likely environmental and hereditarycauses of cancer, there is a need for additional therapeutic modalitiesthat target cancer and related diseases. In particular there is a needfor therapeutic methods for treating diseases associated withdysregulated growth/proliferation.

Cancer is a complex disease arising after a selection process for cellswith acquired functional capabilities like enhanced survival/resistancetowards apoptosis and a limitless proliferative potential. Thus, it ispreferred to develop drugs for cancer therapy addressing distinctfeatures of established tumors.

The PI3K/AKT/mTOR pathway, which is constitutively activated in manytypes of cancers, is one of the prominent pathway that promote tumorcell survival. Initial activation of the PI3K/AKT/mTOR pathway occurs atthe cell membrane, where the signal for pathway activation is propagatedthrough class IA PI3K. Activation of PI3K can occur through tyrosinekinase growth factor receptors (e.g. platelet-derived growth factorreceptor (PDGF-R), human epidermal growth factor 1/2/3 receptor (EGFR,HER2/3), or the insulin-like growth factor 1 receptor (IGF-1R)), celladhesion molecules through integrin-linked kinase (ILK),Ca2+/calmodulin-dependent kinase kinase (CaMKK), nuclear DNA-dependentprotein kinase (DNA-PK), G-protein-coupled receptors, and oncogenicproteins, such as Ras. Once PI3K is activated, it catalyzesphosphorylation of the D-3 position on phosphoinositides to generate thebiologically-active phosphatidylinositol-3,4,5-triphosphate[PI(3,4,5)P₃, PIP₃] and phosphatidylinositol-3,4-bisphosphate[PI(3,4)P₂, PIP₂]. PIP₃ binds to the pleckstrin homology (PH) domains ofphosphoinositide-dependent kinase 1 (PDK-1), AKT, and other PH-domaincontaining proteins, such as Rho and PLC. As the consequence of bindingto PIP₃, the proteins are translocated to the cell membrane and aresubsequently activated. The tumour suppressor PTEN (phosphatase andtensin homolog deleted on chromosome 10) antagonizes PI3K bydephosphorylating PIP₃, thereby preventing translocation and activationof PDK1, AKT and other signaling proteins.^(1,2)

AKT is the major effecter of PI3K, which elicits a broad range ofdownstream signaling events. It recognizes and phosphorylates theconsensus sequence RXRXX(S/T) when surrounded by hydrophobic residues.As this sequence is present in many proteins, about 50 AKT substrateshave been identified and validated.^(3,4) These substrates control keycellular processes such as apoptosis, cell cycle progression,transcription, and translation, stress adaptation, metabolism, andmetastasis of tumor cells. For instance, AKT phosphorylates the FOXOsubfamily of forkhead family transcription factors, which inhibitstranscription of several pro-apoptotic genes, e.g. Fas-L, IGFBP1 andBim.^(5, 6) Additionally, AKT can directly regulate apoptosis byphosphorylating and inactivating pro-apoptotic proteins such as Bad,which control the release of cytochrome c from mitochondria, andapoptosis signal-regulating kinase-1, a mitogen-activated protein kinasekinase involved in stress-induced and cytokine-induced cell death.⁷ Incontrast, AKT can phosphorylate IκB kinase, which indirectly increasesthe activity of nuclear factor KB and stimulates the transcription ofpro-survival genes.⁸ Cell cycle progression can also be affected at theG1/S transition by AKT through its inhibitory phosphorylation of thecyclin dependent kinase inhibitors, p21WAF1/CIP1 and p27KIP1. Inaddition AKT can phosphorylate mouse double minute 2 (MDM2) leading toits nuclear translocation and promotion of degradation of p53. This inconsequence leads to an decrease in p21Cip1mRNA.⁹ Furthermore AKT hasalso an important function in the control of the G2/M transition by e.g.phosphorylation of Myt1 and FOX03a.^(10,11)

The best-studied downstream substrate of AKT is the serine/threoninekinase mTOR. AKT can directly phosphorylate and activate mTOR, as wellas cause indirect activation of mTOR by phosphorylating and inactivatingTSC2 (tuberous sclerosis complex 2, also called tuberin), which normallyinhibits mTOR through the GTP-binding protein Rheb (Ras homolog enrichedin brain). When TSC2 is inactivated by phosphorylation, the GTPase Rhebis maintained in its GTP-bound state, allowing for increased activationof mTOR. mTOR exists in two complexes: the TORC1 complex, in which mTORis bound to Raptor, and the TORC2 complex, in which mTOR is bound toRictor.¹² In the TORC1 complex, mTOR phosphorylates its downstreameffectors S6 kinase (S6K1) and 4EBP-1. S6K1 can then phosphorylate itssubstrate, a ribosomal protein called S6. 4EBP-1, when phosphorylatedcannot bind effectively to its binding partner, eIF4E. The cumulativeeffect is to increase protein translation, especially of highlystructured, capped mRNA species.¹³ Although mTOR is generally considereda downstream substrate of AKT, mTOR in complex with Rictor can alsophosphorylate AKT at 5473, thereby providing a level of positivefeedback on the pathway.¹⁴ Finally, S6K1 can also regulate the pathwayby catalyzing an inhibitory phosphorylation on insulin receptorsubstrate proteins (IRS). This prevents IRS from activating PI3K, whichindirectly lowers activation of AKT. This feedback pathway is veryimportant for developing PI3K/AKT/mTOR pathway inhibitors, as there-activation of PI3K has to be taken into consideration during theevaluation of the anti-tumor efficacy of the PI3K pathwayinhibitors.^(15,16)

In addition to the well described PI3K/AKT/mTOR axis of the PI3Ksignaling pathway, PI3K, AKT and mTOR also receive and branchdifferential signaling events that are independent from the axis. Forexample, mTOR has the crosstalk with and is activated by MAPK pathwaythrough ERK and RSK regulated phosphorylation of TSC2.¹⁷ There arecollective data describing the AKT/mTOR-independent PI3K-mediatedsignaling events. First of all, PI3K downstream signaling molecule PDK1responses to increased levels of PIP3 and activates not only AKT, butalso a group of AGC kinases comprising S6K, RSK, SGK and PKC isoforms,which play essential roles in regulating tumor cell growth,proliferation, survival and metabolism.¹⁸ Furthermore, many PIK3CAmutant cancer cell lines and human breast tumors exhibit only minimalAKT activation and a diminished reliance on AKT foranchorage-independent growth. Instead, these cells retain robust PDK1activation and membrane localization and exhibit dependency on the PDK1substrate SGK3. SGK3 undergoes PI3K- and PDK1-dependent activation inPIK3CA mutant cancer cells. Thus, PI3K may promote cancer through bothAKT-dependent and AKT-independent mechanisms.¹⁹ In addition to PDK1 andAGC kinases, PI3Ks regulate also other cancer related signaling proteinssuch as PLC, Rac, Rho, ITK and BTK, etc.

In humans, class I PI3K has four isoforms of the p110 catalyticsubunits, p110α, p110β, p110γ and 110δ. p110α and p110β are present inall cell types, while p110δ and p110γ are highly enriched in leukocytes.p110 subunits are divided into a class IA group (p110α, p110β andp110δ), which bind the p85 regulatory subunit, and a class IB group(p110γ), which does not. The p85 regulatory subunits contain Srchomology 2 (SH2) domains and bind phosphorylated tyrosine (pTyr), whichlead to the activation of the class IA p110 catalytic subunits. On theother hand, p110γ is activated directly through G protein coupledreceptors (GPCRs). Recent data indicated that p110

was also activated by GPCRs directly through Gβγ protein.²⁰

The signaling inputs to each class I PI3Ks are diverse and well depictedin genetic analyses. Thus, activation of AKT was impaired inp110α-deficient MEFs upon stimulation by classical RTK ligands (EGF,insulin, IGF-1, and PDGF).²¹ On the other hand, MEFs in which p110β isablated or replaced by a kinase-dead allele of p110β respond normally togrowth factor stimulation via RTKs.²² Instead, p110⋅catalytic activityis actually required for AKT activation in response to GPCR ligands(such as LPA). As such, p110α appears to carry the majority of the PI3Ksignal in classic RTK signaling and is responsible for tumor cellgrowth, proliferation, survival, angiogenesis and metabolism whereasp110β mediates GPCR signaling from mitogens and chemokines and thereforemay regulate tumor cell proliferation, metabolism, inflammation andinvasion.^(23, 24)

Although the differences in signaling outputs from the four class I PI3Kisoforms are still largely unknown, it seems that PI3K13 together withPTEN determines the basal levels of PIP3 in tumor cells, while RTKstimulated elevation of PIP3 is controlled mainly by PI3Kα. Thepotential for differential signaling outputs downstream of specific PI3Kisoforms, in parallel with a possibly more universal Akt activation areyet to be discovered.

Activation of PI3K/AKT kinases promotes increased nutrient uptake,converting cells to a glucose-dependent metabolism that redirects lipidprecursors and amino acids to anabolic processes that support cellgrowth and proliferation. These metabolic phenotype with overactivatedAKT lead to malignancies that display a metabolic conversion to aerobicglycolysis (the Warburg effect). In that respect the PI3K/AKT pathway isdiscussed to be central for survival despite unfavourable growthconditions such as glucose depletion or hypoxia.

A further aspect of the activated PI3K/AKT pathway is to protect cellsfrom programmed cell death (“apoptosis”) and is hence considered totransduce a survival signal. By acting as a modulator of anti-apoptoticsignalling in tumor cells, the PI3K/AKT pathway, particular PI3K itselfis a target for cancer therapy. Activated PI3K/AKT phosphorylates andregulates several targets, e.g. BAD, GSK3 or FKHRL1, that affectdifferent signalling pathways like cell survival, protein synthesis orcell movement. This PI3K/AKT pathway also plays a major part inresistance of tumor cells to conventional anti-cancer therapies.Blocking the PI3K/AKT pathway could therefore simultaneously inhibit theproliferation of tumor cells (e.g. via the inhibition of the metaboliceffect) and sensitize towards pro-apoptotic agents. PI3K inhibitionselectively sensitized tumor cells to apoptotic stimuli like Trail,Campthothecin and Doxorubicin.

The resistance of many types of cancer to chemo- and targetedtherapeutics represents the major hurdle in successful cancer treatment.Cancer cells can escape the effect of most commonly used drugs despitetheir different chemical structure and intracellular targets. Manymechanisms underlying the failure of therapeutic drugs have been wellstudied. Activation of PI3K/AKT pathway plays a key role in differentcellular functions such as growth, migration, survival anddifferentiation. Data accumulated in the last decade have establishedthat this pathway plays also a key role in resistance to both chemo-,radiation- and targeted therapeutics. Collective data describingconstitutive or residual pathway activation in cells that have developedresistance to conventional chemotherapy and radiation, as well as toother targeted therapies such as EGFR antagonism. For example,experiments in doxorubicin-resistant CML cell lines demonstrated highlevels of PI3K/AKT activity; importantly, doxorubicin resistance couldbe overcome by decreasing PI3K/AKT activity. Further experimentalevidence was observed in two pancreatic cancer cell lines in whichdecreased levels of phosphorylated AKT can increase gemcitabine-inducedapoptosis. Synergistic antitumor activity with cisplatin was alsodemonstrated in xenograft models of lung cancer.

The PI3K/AKT pathway is linked to resistance to both chemo- and targetedtherapeutics. The Inhibition of PI3Kβ might present a promising strategyto overcome the resistance to radiation and DNA targeting therapy.Nuclear PI3Kb can induce nuclear AKT phosphorylated on both T308 and5473 in response to either IR or the DNA-damaging agent doxorubicin.

In summary, PI3K plays central role downstream of many cancer relatedsignaling pathways that are critical for tumorigenesis, tumorgrowth/proliferation and survival, tumor cell adhesion, invation andmetastasis, as well as tumor angiogenesis. In addition, gain-functionmutation of PIK3CA is common in several human cancers and the linkbetween tumor suppressor gene PTEN and PI3K13 has been observed in sometumors such as prostate cancer. An increased expression of the p110β andp110 δ isoforms has been observed in some colon and bladder tumors, andin glioblastoma. In addition, nuclear PI3Kβ plays roles in DNA synthesisand repair.³⁵ Furthermore, p110δ controls proliferation in acute myeloidleukemia (AML) and migration of breast cancer cells,³⁶ whereas p110γplays roles in tumor angiogenesis, drug resistance of CML cells, andpancreatic tumor growth and survival.³⁷ Thus, developing PI3K inhibitorsfor treatment in mono- and combination therapy is a promising strategyto treat cancer and overcome cancer treatment resistance.

Thus inhibitors of PI3K represent valuable compounds that shouldcomplement therapeutic options not only as single agents but also incombination with other drugs, e.g. DNA targeting agent and radiationtherapy.

Alpharadin (Xofigo) uses alpha radiation from radium-223 decay to killcancer cells. Alpharadin targets to bone tissue by virtue of itschemical similarity to calcium. It has an effect over a range of 2-10cells and causes less damage to surrounding healthy tissues compared tocurrent radiation therapy based on beta or gamma radiation. Significantincrease in median overall survival was demonstrated in Phase IIIclinical trials and Alpharadin (Xofigo) was approved as a treatment forcastration-resistant prostate cancer (CRPC) patients with symptomaticbone metastases.

Different PI3K inhibitors are disclosed in e.g. WO2008/070150,WO2012/062743, WO2012/062745, WO2012/062748.

However, the state of the art does not disclose the combinations of thepresent invention comprising an inhibitor of PI3K kinase or aphysiologically acceptable salt thereof and a pharmaceuticallyacceptable salt of the alkaline-earth radionuclide radium-223.

A preferred suitable pharmaceutically acceptable salt of radium-223 isthe dichloride (Ra²²³Cl₂).

Radium-223 dichloride is a novel, targeted alpha-emitter thatselectively binds to areas of increased bone turnover in bone metastasesand emits high-energy alpha-particles of extremely short (<100 μm)range³⁷

It is the first targeted alpha-emitter approved for the treatment ofprostate cancer with bone metastasis.

As a bone-seeking calcium mimetic, radium-223 is bound into newly formedbone stroma, especially within the microenvironment of osteoblastic orsclerotic metastases.³⁸

The high-energy alpha-particle radiation induces mainly double-strandDNA breaks resulting in a potent and highly localized cytotoxic effectin the target areas containing metastatic cancer cells.³⁹

The short path length of the alpha-particles also means that toxicity toadjacent healthy tissue and particularly the bone marrow may bereduced.⁴⁰

Radium-223 has demonstrated a favorable safety profile, with minimalmyelotoxicity, in phase 1 and 2 studies of patients with bonemetastases.⁴¹

Phase 2 studies have shown that radium-223 reduces pain, improvesdisease-related biomarkers (e.g., bone alkaline phosphatase [ALP] andprostate-specific antigen [PSA]), and have suggested a survival benefitin patients with CRPC and bone metastases.^(42,43) The ALSYMPCA(ALpharadin in SYMptomatic Prostate CAncer patients) trial providesproof of principle for the role of targeted alpha-emitters in oncology.In this trial, radium-223 significantly prolonged overall survival witha 30.5% reduction in risk of death compared with placebo in patientswith CRPC (Castration Resistant Prostate Cancer) and bone metastases.Median survival with radium-223 was longer than placebo by 2.8 months.All main secondary efficacy endpoints were statistically significant andfavored treatment with radium-223, including the clinically definedendpoint of time to first skeletal-related event, which wassignificantly prolonged in patients receiving radium-223.

A substantial percentage of cancer patients is affected by skeletalmetastases. As many as 85% of patients with advanced lung, prostate andbreast carcinoma develop bony metastases.⁴⁴ Bone metastases:Pathophysiology and management policy.). Established treatments such ashormone therapy, chemotherapy and external radiotherapy often causestemporary responses, but ultimately most bone cancer patients experiencerelapses.⁴⁵ There is thus a strong need for new therapies to relievepain and slow down tumor progression.

²²³Ra is used as an α-emitting radiopharmaceutical for targeting ofcalcified tissues, e.g., bone surfaces and osseous tumor lesions. It canbe suitable as a bone seeking radiopharmaceutical.

It thus may be used for prophylactic cancer treatment by delivering afocused dose to bone surfaces in patients with a high probability ofhaving undetected micrometastases at bone surfaces. Another example ofits potential use would be in the treatment of painful osseous sites.

The alkaline-earth radionuclide radium-223 is useful for the targetingof calcified tissues, e.g., bone and a physiological acceptable solutioncomprising ²²³Ra.

The alkaline-earth radionuclide radium-223 is suitable for the use ofthe nuclide as a cationic species and/or associated to a chelator oranother form of a carrier molecule with affinity for calcified tissues.Thus may be combined with a chelator that can be subsequently conjugatedto a molecule with affinity for calcified tissues. The effect of theradioisotope to generated by providing a cascade of α-particles on bonesurfaces and/or in calcified tumors for the palliation of pain caused byvarious diseases and/or for the prophylactic use against possibleminimal disease to the skeleton, and/or also for the therapeutictreatment of established cancer to the bone. The diseases where theradioisotopes could be used includes, but are not limited to skeletalmetastases of prostate-, breast-, kidney- and lung cancer as well asprimary bone cancer and also multiple myeloma.

SUMMARY OF THE INVENTION

Surprisingly it was observed that by administering a PI3K inhibitor or aphysiologically acceptable salt, solvate, hydrate or stereoisomerthereof in combination with a suitable pharmaceutically acceptable saltof the alkaline-earth radionuclide radium-223 a synergisticanti-proliferative and apoptotic effects in breast and prostate tumorcell lines.

Therefore, in accordance with a first aspect, the present inventionprovides combinations of at least two components, component A andcomponent B, component A being an inhibitor of PI3K-kinase (claim 1) ora physiologically acceptable salt, solvate, hydrate or stereoisomerthereof, and component B being a suitable pharmaceutically acceptablesalt of the alkaline-earth radionuclide radium-223.

In accordance with a second aspect, (claim 2) the present inventioncovers combinations of at least two components A and B, component Abeing an inhibitor of PI3K-kinase, and component B being suitablepharmaceutically acceptable salt of the alkaline-earth radionuclideradium-223.

In accordance with a third aspect, (claim 3) the present inventioncomprises combinations of at least two components A and B, component Abeing an inhibitor of PI3K-kinase or a physiologically acceptable saltthereof, and component B being a suitable pharmaceutically acceptableanorganic salt of the alkaline-earth radionuclide radium-223.

The combinations comprising at least two components A and B, asdescribed and defined herein, are also referred to as “combinations ofthe present invention”.

Further, the present invention relates to:

a kit comprising:

-   -   a combination of:

-   Component A: one or more PI3K-kinase inhibitors as described supra    and infra, or a physiologically acceptable salt, solvate, hydrate or    stereoisomer thereof;

-   Component B: a suitable pharmaceutically acceptable salt of the    alkaline-earth radionuclide radium-223 or a solvate or a hydrate    thereof; and, optionally,

-   Component C: one or more further pharmaceutical agents;    in which optionally either or both of said components A and B in any    of the above-mentioned combinations are in the form of a    pharmaceutical formulation/composition which is ready for use to be    administered simultaneously, concurrently, separately or    sequentially. The components may be administered independently of    one another by the oral, intravenous, topical, local installations,    intraperitoneal or nasal route.

In accordance with another aspect, the present invention covers thecombinations as described supra for the treatment or prophylaxis of adisease.

In accordance with another aspect, the present invention covers the useof such combinations as described supra for the preparation of amedicament for the treatment or prophylaxis of a disease.

DETAILED DESCRIPTION OF THE INVENTION Definitions

The terms as mentioned in the present text have preferably the followingmeanings:

-   -   The term ‘alkyl’ refers to a straight or branched hydrocarbon        chain radical consisting solely of carbon and hydrogen atoms,        containing solely of carbon and hydrogen atoms, containing no        unsaturation, having from one to eight carbon atoms, and which        is attached to the rest of the molecule by a single bond, such        as illustratively, methyl, ethyl, n-propyl 1-methylethyl        (isopropyl), n-butyl, n-pentyl, and 1,1-dimethylethyl (t-butyl).    -   The term “alkenyl” refers to an aliphatic hydrocarbon group        containing a carbon-carbon double bond and which may be a        straight or branched or branched chain having about 2 to about        10 carbon atoms, e.g., ethenyl, 1-propenyl, 2-propenyl (allyl),        iso-propenyl, 2-methyl-1-propenyl, 1-butenyl, 2- and butenyl.    -   The term “alkynyl” refers to a straight or branched chain        hydrocarbonyl radicals having at least one carbon-carbon triple        bond, and having in the range of about 2 up to 12 carbon atoms        (with radicals having in the range of about 2 up to 10 carbon        atoms presently being preferred) e.g., ethynyl.    -   The term “alkoxy” denotes an alkyl group as defined herein        attached via oxygen linkage to the rest of the molecule.        Representative examples of those groups are methoxy and ethoxy.    -   The term “alkoxyakyl” denotes an alkoxy group as defined herein        attached via oxygen linkage to an alkyl group which is then        attached to the main structure at any carbon from alkyl group        that results in the creation of a stable structure the rest of        the molecule. Representative examples of those groups are        —CH₂OCH₃, —CH₂OC₂H₅.    -   The term “cycloalkyl” denotes a non-aromatic mono or multicyclic        ring system of about 3 to 12 carbon atoms such as cyclopropyl,        cyclobutyl, cyclopentyl, cyclohexyl and examples of multicyclic        cycloalkyl groups include perhydronapththyl, adamantyl and        norbornyl groups bridged cyclic group or sprirobicyclic groups        e.g sprio (4,4) non-2-yl.    -   The term “cycloalkylalkyl” refers to cyclic ring-containing        radicals containing in the range of about about 3 up to 8 carbon        atoms directly attached to alkyl group which is then also        attached to the main structure at any carbon from the alkyl        group that results in the creation of a stable structure such as        cyclopropylmethyl, cyclobuyylethyl, cyclopentylethyl.    -   The term “aryl” refers to aromatic radicals having in the range        of 6 up to 14 carbon atoms such as phenyl, naphthyl,        tetrahydronapthyl, indanyl, biphenyl.    -   The term “arylalkyl” refers to an aryl group as defined herein        directly bonded to an alkyl group as defined herein which is        then attached to the main structure at any carbon from alkyl        group that results in the creation of a stable structure the        rest of the molecule. e.g., —CH₂C₆H₅, —C₂H₅C₆H₅.    -   The term “heterocyclic ring” refers to a stable 3- to 15        membered ring radical which consists of carbon atoms and from        one to five heteroatoms selected from the group consisting of        nitrogen, phosphorus, oxygen and sulfur. For purposes of this        invention, the heterocyclic ring radical may be a monocyclic,        bicyclic or tricyclic ring system, which may include fused,        bridged or spiro ring systems, and the nitrogen, phosphorus,        carbon, oxygen or sulfur atoms in the heterocyclic ring radical        may be optionally oxidized to various oxidation states. In        addition, the nitrogen atom may be optionally quaternized; and        the ring radical may be partially or fully saturated (i.e.,        heteroaromatic or heteroaryl aromatic). Examples of such        heterocyclic ring radicals include, but are not limited to,        azetidinyl, acridinyl, benzodioxolyl, benzodioxanyl,        benzofurnyl, carbazolyl cinnolinyl dioxolanyl, indolizinyl,        naphthyridinyl, perhydroazepinyl, phenazinyl, phenothiazinyl,        phenoxazinyl, phthalazil, pyridyl, pteridinyl, purinyl,        quinazolinyl, quinoxalinyl, quinolinyl, isoquinolinyl,        tetrazoyl, imidazolyl tetrahydroisouinolyl, piperidinyl,        piperazinyl, 2-oxopiperazinyl, 2-oxopiperidinyl,        2-oxopyrrolidinyl, 2-oxoazepinyl, azepinyl, pyrrolyl,        4-piperidonyl, pyrrolidinyl, pyrazinyl, pyrimidinyl pyridazinyl,        oxazolyl oxazolinyl oxasolidinyl, triazolyl, indanyl,        isoxazolyl, isoxasolidinyl, morpholinyl, thiazolyl, thiazolinyl,        thiazolidinyl, isothiazolyl, quinuclidinyl, isothiazolidinyl,        indolyl, isoindolyl, indolinyl, isoindolinyl, octahydroindolyl,        octahydroisoindolyl quinolyl, isoquinolyl, decahydroisoquinolyl,        benzimidazolyl, thiadiazolyl, benzopyranyl, benzothiazolyl,        benzooxazolyl, furyl, tetrahydrofurtyl, tetrahydropyranyl,        thienyl, benzothienyl, thiamorpholinyl, thiamorpholinyl        sulfoxide thiamorpholinyl sulfone, dioxaphospholanyl,        oxadiazolyl, chromanyl, isochromanyl.    -   The term “heteroaryl” refers to heterocyclic ring radical as        defined herein which are aromatic. The heteroaryl ring radical        may be attached to the main structure at any heteroatom or        carbon atom that results in the creation of a stable structure.    -   The heterocyclic ring radical may be attached to the main        structure at any heteroatom or carbon atom that results in the        creation of a stable structure.    -   The term “heteroarylalkyl” refers to heteroaryl ring radical as        defined herein directly bonded to alkyl group. The        heteroarylalkyl radical may be attached to the main structure at        any carbon atom from alkyl group that results in the creation of        a stable structure.    -   The term “heterocyclyl” refers to a heterocylic ring radical as        defined herein. The heterocylyl ring radical may be attached to        the main structure at any heteroatom or carbon atom that results        in the creation of a stable structure.    -   The term “heterocyclylalkyl” refers to a heterocylic ring        radical as defined herein directly bonded to alkyl group. The        heterocyclylalkyl radical may be attached to the main structure        at carbon atom in the alkyl group that results in the creation        of a stable structure.    -   The term “carbonyl” refers to an oxygen atom bound to a carbon        atom of the molecule by a double bond.    -   The term “halogen” refers to radicals of fluorine, chlorine,        bromine and iodine.

The term “optionally substituted” means optional substitution with thespecified groups, radicals or moieties.

Ring system substituent means a substituent attached to an aromatic ornonaromatic ring system which, for example, replaces an availablehydrogen on the ring system.

As used herein, the term “one or more times”, e.g. in the definition ofthe substituents of the compounds of the present invention (e.g.component A, B or C), is understood as meaning “one, two, three, four orfive times, particularly one, two, three or four times, moreparticularly one, two or three times, even more particularly one or twotimes”.

Where the plural form of the word compounds, salts, polymorphs,hydrates, solvates and the like, is used herein, this is taken to meanalso a single compound, salt, polymorph, isomer, hydrate, solvate or thelike.

By “stable compound’ or “stable structure” is meant a compound that issufficiently robust to survive isolation to a useful degree of purityfrom a reaction mixture, and formulation into an efficacious therapeuticagent.

The term “carbonyl” refers to an oxygen atom bound to a carbon atom ofthe molecule by a double bond.

The compounds of this invention may contain one or more asymmetriccenters, depending upon the location and nature of the varioussubstituents desired. Asymmetric carbon atoms may be present in the (R)-and/or (S)-configuration, resulting in racemic mixtures in the case of asingle asymmetric center, and diastereomeric mixtures in the case ofmultiple asymmetric centers. In certain instances, asymmetry may also bepresent due to restricted rotation about a given bond, for example, thecentral bond adjoining two substituted aromatic rings of the specifiedcompounds. Substituents on a ring may also be present in either cis ortrans form. It is intended that all such configurations (includingenantiomers and diastereomers), are included within the scope of thepresent invention. Preferred compounds are those, which produce the moredesirable biological activity. Separated, pure or partially purifiedisomers and stereoisomers or racemic or diastereomeric mixtures of thecompounds of this invention are also included within the scope of thepresent invention. The purification and the separation of such materialscan be accomplished by standard techniques known in the art.

Tautomers, sometimes referred to as proton-shift tautomers, are two ormore compounds that are related by the migration of a hydrogen atomaccompanied by the switch of one or more single bonds and one or moreadjacent double bonds. The compounds of this invention may exist in oneor more tautomeric forms. For example, a compound of Formula I may existin tautomeric form Ia, tautomeric form Ib, or tautomeric form Ic, or mayexist as a mixture of any of these forms. It is intended that all suchtautomeric forms are included within the scope of the present invention.

The present invention also relates to useful forms of the compounds asdisclosed herein, such as pharmaceutically acceptable salts,co-precipitates, metabolites, hydrates, solvates and prodrugs of all thecompounds of examples. The term “pharmaceutically acceptable salt”refers to a relatively non-toxic, inorganic or organic acid additionsalt of a compound of the present invention. For example, see S. M.Berge, et al. “Pharmaceutical Salts,” J. Pharm. Sci. 1977, 66, 1-19.Pharmaceutically acceptable salts include those obtained by reacting themain compound, functioning as a base, with an inorganic or organic acidto form a salt, for example, salts of hydrochloric acid, sulfuric acid,phosphoric acid, methane sulfonic acid, camphor sulfonic acid, oxalicacid, maleic acid, succinic acid and citric acid. Pharmaceuticallyacceptable salts also include those in which the main compound functionsas an acid and is reacted with an appropriate base to form, e.g.,sodium, potassium, calcium, magnesium, ammonium, and chorine salts.Those skilled in the art will further recognize that acid addition saltsof the claimed compounds may be prepared by reaction of the compoundswith the appropriate inorganic or organic acid via any of a number ofknown methods. Alternatively, alkali and alkaline earth metal salts ofacidic compounds of the invention are prepared by reacting the compoundsof the invention with the appropriate base via a variety of knownmethods.

Representative salts of the compounds of this invention include theconventional non-toxic salts and the quaternary ammonium salts which areformed, for example, from inorganic or organic acids or bases by meanswell known in the art. For example, such acid addition salts includeacetate, adipate, alginate, ascorbate, aspartate, benzoate,benzenesulfonate, bisulfate, butyrate, citrate, camphorate,camphorsulfonate, cinnamate, cyclopentanepropionate, digluconate,dodecylsulfate, ethanesulfonate, fumarate, glucoheptanoate,glycerophosphate, hemisulfate, heptanoate, hexanoate, chloride, bromide,iodide, 2-hydroxyethanesulfonate, itaconate, lactate, maleate,mandelate, methanesulfonate, 2-naphthalenesulfonate, nicotinate,nitrate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate,picrate, pivalate, propionate, succinate, sulfonate, sulfate, tartrate,thiocyanate, tosylate, and undecanoate.

Base salts include alkali metal salts such as potassium and sodiumsalts, alkaline earth metal salts such as calcium and magnesium salts,and ammonium salts with organic bases such as dicyclohexylamine andN-methyl-D-glucamine. Additionally, basic nitrogen containing groups maybe quaternized with such agents as lower alkyl halides such as methyl,ethyl, propyl, or butyl chlorides, bromides and iodides; dialkylsulfates like dimethyl, diethyl, dibutyl sulfate, or diamyl sulfates,long chain halides such as decyl, lauryl, myristyl and strearylchlorides, bromides and iodides, aralkyl halides like benzyl andphenethyl bromides and others.

A solvate for the purpose of this invention is a complex of a solventand a compound of the invention in the solid state. Exemplary solvateswould include, but are not limited to, complexes of a compound of theinvention with ethanol or methanol. Hydrates are a specific form ofsolvate wherein the solvent is water.

Constituents which are optionally substituted as stated herein, may besubstituted, unless otherwise noted, one or more times, independentlyfrom one another at any possible position. When any variable occurs morethan one time in any constituent, each definition is independent.

The heteroarylic, or heterocyclic groups mentioned herein can besubstituted by their given substituents or parent molecular groups,unless otherwise noted, at any possible position, such as e.g. at anysubstitutable ring carbon or ring nitrogen atom. Analogously it is beingunderstood that it is possible for any heteroaryl or heterocyclyl groupto be attached to the rest of the molecule via any suitable atom ifchemically suitable. Unless otherwise noted, any heteroatom of aheteroarylic ring with unsatisfied valences mentioned herein is assumedto have the hydrogen atom(s) to satisfy the valences. Unless otherwisenoted, rings containing quaternizable amino- or imino-type ring nitrogenatoms (—N═) may be preferably not quaternized on these amino- orimino-type ring nitrogen atoms by the mentioned substituents or parentmolecular groups.

Preferred compounds are those which produce the more desirablebiological activity. Separated, pure or partially purified isomers andstereoisomers or racemic or diastereomeric mixtures of the compounds ofthis invention are also included within the scope of the presentinvention. The purification and the separation of such materials can beaccomplished by standard techniques already known in the art.

The optical isomers can be obtained by resolution of the racemicmixtures according to conventional processes, for example, by theformation of diastereoisomeric salts using an optically active acid orbase or formation of covalent diastereomers. Examples of appropriateacids are tartaric, diacetyltartaric, ditoluoyltartaric andcamphorsulfonic acid. Mixtures of diastereoisomers can be separated intotheir individual diastereomers on the basis of their physical and/orchemical differences by methods known in the art, for example, bychromatography or fractional crystallisation. The optically active basesor acids are then liberated from the separated diastereomeric salts. Adifferent process for separation of optical isomers involves the use ofchiral chromatography (e.g., chiral HPLC columns), with or withoutconventional derivatisation, optimally chosen to maximise the separationof the enantiomers. Suitable chiral HPLC columns are manufactured byDiacel, e.g., Chiracel OD and Chiracel OJ among many others, allroutinely selectable. Enzymatic separations, with or withoutderivatisation, are also useful. The optically active compounds of thisinvention can likewise be obtained by chiral syntheses utilizingoptically active starting materials.

If in the context of the invention “embodiment” is mentioned it shouldbe understood to include a plurality of possible combinations.

In order to limit different types of isomers from each other referenceis made to IUPAC Rules Section E (Pure Appl Chem 45, 11-30, 1976).

The invention also includes all suitable isotopic variations of acompound of the invention. An isotopic variation of a compound of theinvention is defined as one in which at least one atom is replaced by anatom having the same atomic number but an atomic mass different from theatomic mass usually or predominantly found in nature.

Examples of isotopes that can be incorporated into a compound of theinvention include isotopes of hydrogen, carbon, nitrogen, oxygen,phosphorus, sulphur, fluorine, chlorine, bromine and iodine, such as ²H(deuterium), ³H (tritium), ¹¹C, ¹³C, ¹⁴C, ¹⁵N, ¹⁷O, ¹⁸O, ³²P, ³³P, ³³S,³⁴S, ³⁵S, ³⁶S, ¹⁸F, ³⁶Cl, ⁸²Br, ¹²³I, ¹²⁴I, ¹²⁹I and ¹³¹I, respectively.Certain isotopic variations of a compound of the invention, for example,those in which one or more radioactive isotopes such as ³H or ¹⁴C areincorporated, are useful in drug and/or substrate tissue distributionstudies. Tritiated and carbon-14, i.e., ¹⁴C, isotopes are particularlypreferred for their ease of preparation and detectability. Further,substitution with isotopes such as deuterium may afford certaintherapeutic advantages resulting from greater metabolic stability, forexample, increased in vivo half-life or reduced dosage requirements andhence may be preferred in some circumstances. Isotopic variations of acompound of the invention can generally be prepared by conventionalprocedures known by a person skilled in the art such as by theillustrative methods or by the preparations described in the exampleshereafter using appropriate isotopic variations of suitable reagents.

The present invention includes all possible stereoisomers of thecompounds of the present invention as single stereoisomers, or as anymixture of said stereoisomers, in any ratio. Isolation of a singlestereoisomer, e.g. a single enantiomer or a single diastereomer, of acompound of the present invention may be achieved by any suitable stateof the art method, such as chromatography, especially chiralchromatography, for example.

The present invention includes all possible tautomers of the compoundsof the present invention as single tautomers, or as any mixture of saidtautomers, in any ratio.

Furthermore, the present invention includes all possible crystallineforms, or polymorphs, of the compounds of the present invention, eitheras single polymorphs, or as a mixture of more than one polymorphs, inany ratio.

Component A of the Combination

Component A can be selected from inhibitors of PI3K-kinase specificallyor generically disclosed e.g. in the publications as mentioned abovewhich are incorporated herein by reference.

In an embodiment, said component A is a compound of general formula (A):

in which:X represents CR⁵R⁶ or NH;Y¹ represents CR³ or N;the chemical bond between Y²═Y³ represents a single bond or double bond,with the proviso that when the Y²═Y³ represents a double bond, Y² and Y³independently represent CR⁴ or N, andwhen Y²═Y³ represents a single bond, Y² and Y³ independently representCR³R⁴ or NR⁴;Z¹, Z², Z³ and Z⁴ independently represent CH, CR² or N;

-   -   R¹ represents aryl optionally having 1 to 3 substituents        selected from R¹¹, C₃₋₈ cycloalkyl optionally having 1 to 3        substituents selected from R¹¹, C₁₋₆ alkyl optionally        substituted by aryl, heteroaryl, C₁₋₆ alkoxyaryl, aryloxy,        heteroaryloxy or one or more halogen,        -   C₁₋₆ alkoxy optionally substituted by carboxy, aryl,            heteroaryl, C₁₋₆ alkoxyaryl, aryloxy, heteroaryloxy or one            or more halogen,        -   or        -   a 3 to 15 membered mono- or bi-cyclic heterocyclic ring that            is saturated or unsaturated, optionally having 1 to 3            substituents selected from R¹¹, and contains 1 to 3            heteroatoms selected from the group consisting of N, O and            S,        -   wherein        -   R¹¹ represents halogen, nitro, hydroxy, cyano, carboxy,            amino, N—(C₁₋₆ alkyl)amino, N-(hydroxyC₁₋₆ alkyl)amino,            N,N-di(C₁₋₆ alkyl)amino, N—(C₁₋₆ acyl)amino,            N-(formyl)-N—(C₁₋₆ alkyl)amino, N—(C₁₋₆ alkanesulfonyl)            amino, N-(carboxyC₁₋₆ alkyl)-N—(C₁₋₆ alkyl)amino,            N—(C₁₋₆alkoxycabonyl)amino, N—[N,N-di(C₁₋₆ alkyl)amino            methylene]amino, N—[N,N-di(C₁₋₆ alkyl)amino (C₁₋₆            alkyl)methylene]amino, N—[N,N-di(C₁₋₆alkyl)amino C₂₋₆            alkenyl]amino, aminocarbonyl, N—(C₁₋₆ alkyl)aminocarbonyl,            N,N-di(C₁₋₆ alkyl)aminocarbonyl, C₃₋₈ cycloalkyl, C₁₋₆            alkylthio, C₁₋₆ alkanesulfonyl, sulfamoyl, C₁₋₆            alkoxycarbonyl, N-arylamino wherein said aryl moiety is            optionally having 1 to 3 substituents selected from R¹⁰¹,            N-(aryl C₁₋₆ alkyl)amino wherein said aryl moiety is            optionally having 1 to 3 substituents selected from R¹⁰¹,            aryl C₁₋₆ alkoxycarbonyl wherein said aryl moiety is            optionally having 1 to 3 substituents selected from R¹⁰¹,        -   C₁₋₆ alkyl optionally substituted by mono-, di- or            tri-halogen, amino, N—(C₁₋₆ alkyl)amino or N,N-di(C₁₋₆            alkyl)amino,        -   C₁₋₆ alkoxy optionally substituted by mono-, di- or            tri-halogen, N—(C₁₋₆ alkyl)sulfonamide, or            N-(aryl)sulfonamide,        -   or        -   a 5 to 7 membered saturated or unsaturated ring having 1 to            3 heteroatoms selected from the group consisting of O, S and            N, and optionally having 1 to 3 substituents selected from            R¹⁰¹        -   wherein        -   R¹⁰¹ represents halogen, carboxy, amino, N—(C₁₋₆            alkyl)amino, N,N-di(C₁₋₆alkyl)amino, aminocarbonyl, N—(C₁₋₆            alkyl)aminocarbonyl, N,N-di(C₁₋₆ alkyl)aminocarbonyl,            pyridyl,            -   C₁₋₆ alkyl optionally substituted by cyano or mono- di-                or tri-halogen,            -   and            -   C₁₋₆ alkoxy optionally substituted by cyano, carboxy,                amino, N—(C₁₋₆ alkyl)amino, N,N-di(C₁₋₆ alkyl)amino,                aminocarbonyl, N—(C₁₋₆alkyl)aminocarbonyl, N,N-di(C₁₋₆                alkyl)aminocarbonyl or mono-, di- or tri-halogen;    -   R² represents hydroxy, halogen, nitro, cyano, amino, N—(C₁₋₆        alkyl)amino, N,N-di(C₁₋₆ alkyl)amino, N-(hydroxyC₁₋₆        alkyl)amino, N-(hydroxyC₁₋₆ alkyl)-N—(C₁₋₆ alkyl)amino, C₁₋₆        acyloxy, aminoC₁₋₆ acyloxy, C₂₋₆ alkenyl, aryl,        -   a 5-7 membered saturated or unsaturated heterocyclic ring            having 1 to 3 heteroatoms selected from the group consisting            O, S and N, and optionally substituted by        -   hydroxy, C₁₋₆ alkyl, C₁₋₆ alkoxy, oxo, amino, amino C₁₋₆            alkyl, N—(C₁₋₆ alkyl)amino, N,N-di(C₁₋₆ alkyl)amino, N—(C₁₋₆            acyl)amino, N—(C₁₋₆ alkyl)carbonylamino, phenyl, phenyl C₁₋₆            alkyl, carboxy, C₁₋₆ alkoxycarbonyl, aminocarbonyl, N—(C₁₋₆            alkyl)aminocarbonyl, or N,N-di(C₁₋₆ alkyl)amino, —C(O)—R²⁰        -   wherein        -   R²⁰ represents C₁₋₆ alkyl, C₁₋₆ alkoxy, amino,            N—(C₁₋₆alkyl)amino, N,N-di(C₁₋₆ alkyl)amino, N—(C₁₋₆            acyl)amino, or a 5-7 membered saturated or unsaturated            heterocyclic ring having 1 to 3 heteroatoms selected from            the group consisting O, S and N, and optionally substituted            by C₁₋₆ alkyl, C₁₋₆ alkoxy, oxo, amino, N—(C₁₋₆alkyl)amino,            N,N-di(C₁₋₆ alkyl)amino, N—(C₁₋₆ acyl)amino, phenyl, or            benzyl,            -   C₁₋₆ alkyl optionally substituted by R²¹,            -   or            -   C₁₋₆ alkoxy optionally substituted by R²¹,            -   wherein            -   R²¹ represents cyano, mono-, di or tri-halogen, amino,                N—(C₁₋₆alkyl)amino, N,N-di(C₁₋₆ alkyl)amino,                N-(hydroxyC₁₋₆ alkyl) amino, N-(halophenylC₁₋₆ alkyl)                amino, amino C₂₋₆ alkylenyl, C₁₋₆ alkoxy, hydroxyC₁₋₆                alkoxy, —C(O)—R²⁰¹, —NHC(O)—R²⁰¹, C₃₋₈ cycloalkyl,                isoindolino, phthalimidyl, 2-oxo-1,3-oxazolidinyl, aryl                or a 5 or 6 membered saturated or unsaturated                heterocyclic ring having 1 to 4 heteroatoms selected                from the group consisting 0, S and N, and optionally                substituted by hydroxy, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆                alkoxycarbonyl, hydroxyC₁₋₆ alkoxy, oxo, amino,                aminoC₁₋₆ alkyl, N—(C₁₋₆ alkyl)amino, N,N-di(C₁₋₆                alkyl)amino, N—(C₁₋₆ acyl)amino, or benzyl,            -   wherein            -   R²⁰¹ represents hydroxy, amino, N—(C₁₋₆ alkyl)amino,                N,N-di(C₁₋₆ alkyl)amino, N-(halophenylC₁₋₆ alkyl) amino,                C₁₋₆ alkyl, aminoC₁₋₆ alkyl, aminoC₂₋₆ alkylenyl, C₁₋₆                alkoxy, a 5 or 6 membered saturated or unsaturated                heterocyclic ring having 1 to 4 heteroatoms selected                from the group consisting O, S and N, and optionally                substituted by hydroxy, C₁₋₆ alkyl, C₁₋₆ alkoxy, C₁₋₆                alkoxycarbonyl, hydroxyC₁₋₆ alkoxy, oxo, amino, N—(C₁₋₆                alkyl)amino, N,N-di(C₁₋₆ alkyl)amino, N—(C₁₋₆ acyl)amino                or benzyl;    -   R³ represents hydrogen, halogen, aminocarbonyl, or C₁₋₆ alkyl        optionally substituted by aryl C₁₋₆ alkoxy or mono-, di- or        tri-halogen;    -   R⁴ represents hydrogen or C₁₋₆ alkyl;    -   R⁵ represents hydrogen or C₁₋₆ alkyl; and    -   R⁶ represents halogen, hydrogen or C₁₋₆ alkyl,        or a physiologically acceptable salt, solvate, hydrate or        stereoisomer thereof.

In an embodiment, said component A is a compound of general formula (A),supra, which is selected from the list consisting of:

-   N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   2-(7,    8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-pyridin-3-ylethylenol;-   N-(7,    8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   6-(acetamido)-N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-{5-[2-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide;-   2-({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)-N,N-dimethylacetamide;-   2-[7-methoxy-8-(tetrahydro-2H-pyran-2-ylmethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;-   2-[8-(2-hydroxyethoxy)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;-   ({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)acetic    acid;-   4-({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)butanoic    acid;-   ({5-[2-hydroxy-2-pyridin-3-ylvinyl]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-8-yl}oxy)acetonitrile;-   2-[7-methoxy-8-(2H-tetrazol-5-ylmethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethylenol;-   2-[7-methoxy-8-(4-morpholin-4-yl-4-oxobutoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-pyridin-3-ylethyl    enol;-   5-[1-hydroxy-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)vinyl]pyridin-3-ol;-   N-(2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;-   6-(acetamido)-N-(7,9-dimethoxy-8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;    5-hydroxy-N-(7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-[(4-methoxybenzyl)oxy]nicotinamide;-   N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-5-hydroxynicotinamide;-   5-hydroxy-N-[8-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-{8-[3-(1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl)propoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-(7-bromo-8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   6-amino-N-(8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   1-(1H-benzimidazol-5-yl)-2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)ethylenol;-   2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-(2,4-dimethyl-1,3-thiazol-5-yl)ethylenol;-   N-(9-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   N-(8-bromo-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(8-bromo-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   N-(8-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   N-(8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   N-[8-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1H-benzimidazole-5-carboxamide;-   N-(7-fluoro-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   N-(7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(8-chloro-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   6-(acetamido)-N-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   1-(1H-benzimidazol-5-yl)-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)ethylenol;-   N-{5-[1-hydroxy-2-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)vinyl]pyridin-2-yl}acetamide;-   6-methyl-N-(8-morpholin-4-yl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   1-(1H-benzimidazol-5-yl)-2-[8-(4-methylpiperazin-1-yl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]ethylenol;-   N-(2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-3H-imidazo[4,5-b]pyridine-6-carboxamide;-   N-(7,8-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-3H-imidazo[4,5-b]pyridine-6-carboxamide;-   N-[7-(trifluoromethyl)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1H-benzimidazole-5-carboxamide;-   N-(7,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1H-benzimidazole-5-carboxamide;-   N-{5-[2-(7,9-dimethoxy-8-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide;-   N-{5-[2-(7-bromo-9-methyl-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-hydroxyvinyl]pyridin-2-yl}acetamide;    and-   2-(8,9-dimethoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-1-pyridin-3-ylethylenol;

In an embodiment, said component A is a compound having the formula (I):

-   -   or a physiologically acceptable salt, solvate, hydrate or        stereoisomer thereof, in which:

-   R¹ represents —(CH₂)_(n)—(CHR⁴)—(CH₂)_(m)—N(R⁵)(R^(5′));

-   R² represents a heteroaryl optionally substituted with 1, 2 or 3 R⁶    groups;

-   R³ represents alkyl or cycloalkyl;

-   R⁴ represents hydrogen or alkoxy; and

-   R⁵ and R^(5′) may be the same or different and represent    independently, hydrogen, alkyl, cycloalkylalklyl, or alkoxyalkyl or    R⁵ and R^(5′) may be taken together with the nitrogen atom to which    they are bound to form a 3-7 membered nitrogen containing    heterocyclic ring optionally containing at least one additional    heteroatom selected from oxygen, nitrogen or sulfur and which may be    optionally substituted with 1 or more R^(6′) groups, or R⁴ and R⁵    may be taken together with the atoms to which they are bound to form    a 5-6 membered nitrogen containing heterocyclic ring optionally    containing 1 or more nitrogen, oxygen or sulfur atoms and which may    be optionally substituted with 1 or more R^(6′) groups;    each occurrence of R⁶ may be the same or different and is    independently halogen, alkyl, alkenyl, alkynyl, cycloalkyl,    cycloalkylalklyl, aryl, arylalkyl, heteroaryl, heteroarylalkyl,    heterocyclic ring, heterocyclylalkyl, alkyl-OR⁷, alkyl-SR⁷,    alkyl-N(R⁷)(R^(7′)), alkyl-COR⁷, —CN, —COOR⁷, —CON(R⁷)(R^(7′)),    —OR⁷, —SR⁷, —N(R⁷)(R^(7′)), or —NR⁷COR⁷ each of which may be    optionally substituted with 1 or more R⁸ groups;    each occurrence of R^(6′) may be the same or different and is    independently alkyl, cycloalkylalklyl, or alkyl-OR⁷;    each occurrence of R⁷ and R^(7′) may be the same or different and is    independently hydrogen, alkyl, alkenyl, alkynyl, cycloalkyl,    cycloalkylalklyl, cycloalkenyl, aryl, arylalkyl, heteroaryl,    heterocyclic ring, heterocyclylalkyl, or heteroarylalkyl;    each occurrence of R⁸ is independently nitro, hydroxy, cyano,    formyl, acetyl, halogen, amino, alkyl, alkoxy, alkenyl, alkynyl,    cycloalkyl, cycloalkylalklyl, cycloalkenyl, aryl, arylalkyl,    heteroaryl, heterocyclic ring, heterocyclylalkyl, or    heteroarylalkyl;    n is an integer from 1-4 and m is an integer from 0-4 with the    proviso that when when R⁴ and R⁵ are taken together with the atoms    to which they are bound to form a 5-6 membered nitrogen containing    ring, n+m≤4;    or a physiologically acceptable salt, solvate, hydrate or    stereoisomer thereof.

In an embodiment, said component A is a compound having the formula (I),supra, in which R² is a nitrogen containing heteroaryl optionallysubstituted with 1, 2 or 3 R⁶ groups,

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof.

In an embodiment, said component A is a compound of general formula (I),supra, in which R⁵ and R^(5′) are independently alkyl,

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof.

In an embodiment, said component A is a compound of general formula (I),supra, in which R⁵ and R^(5′) are taken together with the nitrogen atomto which they are bound to form a 5-6 membered nitrogen containingheterocyclic ring containing at least one additional heteroatom selectedfrom oxygen, nitrogen or sulfur and which may be optionally substitutedwith 1 or more R^(6′) groups,

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof.

In an embodiment, said component A is a compound of formula (I) in whichR⁴ and R⁵ are taken together with the atoms to which they are bound toform a 5-6 membered nitrogen containing heterocyclic ring optionallycontaining 1 or more nitrogen, oxygen or sulfur atoms and which may beoptionally substituted with 1 or more R⁶ groups, or a physiologicallyacceptable salt, solvate, hydrate or stereoisomer thereof.

In an embodiment, said component A is a compound of formula (I) in whichR³ is methyl,

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof.

In an embodiment, said component A is a compound of formula (I), whereinR² is pyridine, pyridazine, pyrimidine, pyrazine, pyrole, oxazole,thiazole, furan or thiophene, optionally substituted with 1, 2 or 3 R⁶groups; more preferably pyridine, pyridazine, pyrimidine, pyrazine,pyrole, oxazole or thiazole, optionally substituted with 1, 2 or 3 R⁶groups,

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof.

In an embodiment, said component A is a compound of formula (Ia):

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof, wherein R² is as defined above for formula (I).

In an embodiment, said component A is a compound of formula (Ib):

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof, wherein R² is as defined above for formula (I).

In an embodiment, said component A is a compound of formula (Ic):

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof, wherein R² is as defined above for formula (I).

In an embodiment, said component A is a compound of the formula (Id):

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof, wherein R² and R⁴ are as defined above for formula (I).

In an embodiment, said component A is a compound of the formula (Ie):

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof, wherein R² and R⁴ are as defined above for formula (I).

In an embodiment, said component A is a compound of formula (I)-(Ie),wherein R² is pyridine, pyridazine, pyrimidine, pyrazine, pyrole,oxazole, thiazole, furan or thiophene, optionally substituted with 1, 2or 3 R⁶ groups; more preferrably wherein R² is pyridine, pyridazine,pyrimidine, pyrazine, pyrole, oxazole or thiazole, optionallysubstituted with 1, 2 or 3 R⁶ groups,

or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof.

In an embodiment, said component A is a compound selected from the listconsisting of:

-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;-   N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-2,4-dimethyl-1,3-thiazole-5-carboxamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1,3-thiazole-5-carboxamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]isonicotinamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-4-methyl-1,3-thiazole-5-carboxamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-4-propylpyrimidine-5-carboxamide;-   N-{8-[2-(4-ethylmorpholin-2-yl)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-{8-[2-(dimethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}pyrimidine-5-carboxamide;-   N-(8-{3-[2-(hydroxymethyl)    morpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(8-{3-[2-(hydroxymethyl)    morpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-{8-[3-(dimethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide    1-oxide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-(2-pyrrolidin-1-ylethyl)nicotinamide;-   6-(cyclopentylamino)-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[8-(2-hydroxy-3-morpholin-4-ylpropoxy)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-{7-methoxy-8-[3-(3-methylmorpholin-4-yl)propoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-(8-{3-[2-(hydroxymethyl)    morpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(8-{2-[4-(cyclobutylmethyl)    morpholin-2-yl]ethoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-(7-methoxy-8-{2-[4-(2-methoxyethyl)morpholin-2-yl]ethoxy}-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-{8-[(4-ethylmorpholin-2-yl)methoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-(7-methoxy-8-{[4-(2-methoxyethyl)morpholin-2-yl]methoxy}-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-{7-methoxy-8-[(4-methyl    morpholin-2-yl)methoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-4-carboxamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-4-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-methyl-1H-imidazole-4-carboxamide;-   rel-N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)pyrimidine-5-carboxamide;-   rel-N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)-6-methylnicotinamide;-   rel-6-acetamido-N-(8-{3-[(2R,6S)-2,6-dimethyl    morpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1-methyl-1H-imidazole-5-carboxamide;-   6-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-methylnicotinamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-4-methylpyrimidine-5-carboxamide;-   6-amino-5-bromo-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1,3-oxazole-5-carboxamide;-   N-[7-methoxy-8-(morpholin-2-ylmethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   2-{[2-(dimethylamino)ethyl]amino}-N-{8-[3-(dimethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}pyrimidine-5-carboxamide;-   2-amino-N-{8-[3-(dimethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}-1,3-thiazole-5-carboxamide;-   rel-2-amino-N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)pyrimidine-5-carboxamide;-   rel-6-amino-N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   2-[(2-hydroxyethyl)amino]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-[(3-methoxypropyl)amino]pyrimidine-5-carboxamide;-   2-amino-N-{8-[3-(dimethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}pyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-[(3-morpholin-4-ylpropyl)amino]pyrimidine-5-carboxamide;-   2-[(2-methoxyethyl)amino]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;-   2-{[2-(dimethyl    amino)ethyl]amino}-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;-   6-amino-N-{8-[3-(dimethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-pyrrolidin-1-ylpyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-(4-methylpiperazin-1-yl)pyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-morpholin-4-ylpyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-piperazin-1-ylnicotinamide    hydrochloride;-   6-[(3S)-3-aminopyrrolidin-1-yl]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide    hydrochloride hydrate;-   6-[(3R)-3-aminopyrrolidin-1-yl]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide    hydrochloride;-   6-[(4-fluorobenzyl)amino]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   6-[(2-furylmethyl)amino]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   6-[(2-methoxyethyl)amino]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-(1H-pyrrol-1-yl)nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-morpholin-4-ylnicotinamide;-   N-{7-methoxy-8-[3-(methylamino)propoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   6-[(2,2-dimethylpropanoyl)amino]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   6-[(cyclopropylcarbonyl)amino]-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-(2,2,2-trifluoroethoxy)nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-(trifluoromethyl)nicotinamide;-   6-(isobutyrylamino)-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-{7-methoxy-8-[3-(4-methylpiperazin-1-yl)propoxy]-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-{[(methylamino)carbonyl]amino}-1,3-thiazole-4-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-{[(methylamino)carbonyl]amino}nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-(methylamino)-1,3-thiazole-4-carboxamide;-   N-[7-methoxy-8-(2-morpholin-4-ylethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-{8-[2-(dimethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}-2,4-dimethyl-1,3-thiazole-5-carboxamide;-   N-{8-[2-(dimethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}-6-methylnicotinamide;-   6-{[(isopropylamino)carbonyl]amino}-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-pyrrolidin-1-ylnicotinamide;-   6-(dimethylamino)-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-piperidin-1-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(2-pyrrolidin-1-ylethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(2-piperidin-1-ylethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   6-{[(ethylamino)carbonyl]amino}-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   6-fluoro-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1,3-oxazole-4-carboxamide;-   2-(ethylamino)-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-1,3-thiazole-4-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrazine-2-carboxamide;-   N-[8-(2-aminoethoxy)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   6-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]isonicotinamide;-   N-{8-[3-(diethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-{8-[2-(diisopropylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-{8-[2-(diethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-{8-[3-(dimethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-{8-[2-(dimethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-(methylamino)pyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-(methylthio)pyrimidine-5-carboxamide;-   N-[8-(3-aminopropoxy)-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide    trifluoroacetate;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]thiophene-2-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2,4-dimethyl-1,3-thiazole-5-carboxamide;-   2-methoxy-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-3-furamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]thiophene-3-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2-methyl-1,3-thiazole-4-carboxamide;-   6-methoxy-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   5-methoxy-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-methylnicotinamide;-   6-(acetylamino)-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;    or a physiologically acceptable salt, solvate, hydrate or    stereoisomer thereof.

In another embodiment, said component A is a compound selected from thelist consisting of:

-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-6-methylnicotinamide;-   5-methoxy-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]-2,4-dimethyl-1,3-thiazole-5-carboxamide;-   N-{8-[2-(dimethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   N-{8-[3-(dimethylamino)propoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}nicotinamide;-   6-{[(isopropylamino)carbonyl]amino}-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   N-{8-[2-(dimethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}-2,4-dimethyl-1,3-thiazole-5-carboxamide;-   N-[7-methoxy-8-(2-morpholin-4-ylethoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]nicotinamide;-   rel-6-amino-N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)nicotinamide;-   rel-2-amino-N-(8-{3-[(2R,6S)-2,6-dimethylmorpholin-4-yl]propoxy}-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl)pyrimidine-5-carboxamide;-   2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;-   N-{8-[2-(dimethylamino)ethoxy]-7-methoxy-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl}pyrimidine-5-carboxamide;-   N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide;    or a physiologically acceptable salt, solvate, hydrate or    stereoisomer thereof.

In another embodiment, said component A is2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide,or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof.

In another embodiment, said component A is2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamidedihydrochloride.

Where there is a discrepancy between the chemical name and the chemicalstructure depicted, the chemical structure depicted takes precedenceover the chemical name given.

Without being bound by theory or mechanism, the compounds of the presentinvention display surprising activity for the inhibition ofphosphatidylinositol-3-kinase and chemical and structural stability overthose compounds of the prior art. It is believed that this surprisingactivity is based on the chemical structure of the compounds, inparticular the basicity of the compounds as a result of R¹ being aminooptionally substituted with R⁵ and R^(5′). Further, the appropriatechoice of R³ and R² provide the necessary activity against theappropriate isoforms to allow for activity in vivo.

The synthesis of the compounds listed above is described inInternational Patent Application No. PCT/EP2003/010377, published as WO2004/029055 A1, and in International Patent Application No.PCT/US2007/024985, published as WO 2008/070150, both of which are herebyincorporated herein in their entirety by reference.

Said component A may be in the form of a pharmaceutical formulationwhich is ready for use to be administered simultaneously, concurrently,separately or sequentially. The components may be administeredindependently of one another by the oral, intravenous, topical, localinstallations, intraperitoneal or nasal route.

The PI3K-inhibitors mentioned in the prior art as well as in the listsabove have been disclosed for the treatment or prophylaxis of differentdiseases, especially cancer.

The specific compounds of the lists as disclosed above are preferred asbeing component A of the combination, most preferred is the compoundused in the experimental section.

The synergistic behavior of a combination of the present invention isdemonstrated herein with one of the PI3K inhibitors specificallydisclosed in the Examples section as compound A.

In addition a combination of the present invention comprising compound Aas mentioned above and a pharmaceutically acceptable salt of radium-223is a preferred aspect of the invention.

In another aspect a combination of the present invention comprisescompound A or a pharmaceutically acceptable salt thereof as mentionedabove and a pharmaceutically acceptable salt of the alkaline-earthradionuclide radium-223.

In another aspect a combination of the present invention comprisescompound A or a pharmaceutically acceptable salt thereof as mentionedabove and the dichloride salt of radium-223.

It is to be understood that the present invention relates also to anycombination of the embodiments of component A described above.

Component B of the Combination

Component B is a suitable pharmaceutically acceptable salt of thealkaline-earth radionuclide radium-223.

A suitable pharmaceutically acceptable salt of radium-223 can be, forexample, an acid addition salt with an inorganic acid, such ashydrochloric, hydrobromic, hydroiodic, sulfuric, bisulfuric, phosphoric,or nitric acid, for example, or with an organic acid, such as formic,acetic, acetoacetic, pyruvic, trifluoroacetic, propionic, butyric,hexanoic, heptanoic, undecanoic, lauric, benzoic, salicylic, 2 (4hydroxybenzoyl) benzoic, camphoric, cinnamic, cyclopentanepropionic,digluconic, 3 hydroxy 2 naphthoic, nicotinic, pamoic, pectinic,persulfuric, 3 phenylpropionic, picric, pivalic, 2hydroxyethanesulfonate, itaconic, sulfamic, trifluoromethanesulfonic,dodecylsulfuric, ethansulfonic, benzenesulfonic, para toluenesulfonic,methansulfonic, 2 naphthalenesulfonic, naphthalinedisulfonic,camphorsulfonic acid, citric, tartaric, stearic, lactic, oxalic,malonic, succinic, malic, adipic, alginic, maleic, fumaric, D gluconic,mandelic, ascorbic, glucoheptanoic, glycerophosphoric, aspartic,sulfosalicylic, hemisulfuric, or thiocyanic acid, for example.

A preferred suitable pharmaceutically acceptable salt of radium-223 isthe dichloride (Ra²²³Cl₂).

Methods for preparation of a physiologically acceptable solutioncomprising radium-223 are disclosed e.g. in WO 2000/40275(A2), WO2011/134671(A1), and WO 2011/134672(A1).

Physiologically acceptable solutions comprising radium-223 show a uniquemechanism of action as a targeted radiopharmaceutical. They represent anew generation of alpha emitting therapeutic pharmaceuticals based onthe natural bone-seeking nuclide radium-223.

Preferably, an aqueous solution of radium-223 chloride (²²³RaCl2) forintravenous injection, sterile and free from bacterial endotoxins isused.

Preferably, the solution is isotonic, containing a sodium citratebuffered saline to physiological pH.

A preferred dosage regimen for radium-223 chloride injection is 50 kBqper kg body weight given at 4 week intervals, as a course consisting of6 injections. Single radium-223 doses up to 250 kBq per kg body weightwere evaluated in a phase I clinical trial.

The observed adverse reactions at this dose were diarrhea and reversiblemyelosuppression (including one case (⅕) of grade 3 neutropenia).

As an example, the aqueous radium-223 dichloride solution may besupplied in a single-dose 10 ml vial which contains a fill volume of 6ml. This product has a radioactivity concentration of radium-223 of1,000 kBq/mL (0.03 mCi/mL), corresponding to 0.53 ng/mL of radium atreference date. The active moiety is the alpha particle emitting nuclideradium 223 (half-life is 11.4 days), present as a divalent cation(²²³Ra²⁺). The fraction of energy emitted from radium-223 and itsdaughters as alpha-particles is 95.3%, the fraction emitted asbeta-particles is 3.6%, and the fraction emitted as gamma-radiation is1.1%. The combined energy from the emitted radiation from complete decayof radium-223 and its daughter nuclides is 28.2 MeV.

Radium-223 is to be administered intravenously by qualified personnel asa slow bolus injection. An intravenous access line should be used foradministration of Radium-223. The line must be flushed with isotonicsaline before and after injection of Radium-223.

Radium-223 selectively targets areas of increased bone turnover, as inbone metastases, and concentrates by forming a complex withhydroxyapatite. Alpha emission contributes about 93% of the totalradiation absorbed dose. The high linear energy alpha particle radiationinduces double-strand DNA breaks, resulting in a potent and localizedcytotoxic effect in the target areas containing metastatic cancer cells.The short path length (less than 100 micrometers) of the alpha particlesminimizes the effect on adjacent healthy tissue such as the bone marrow.

In accordance with an embodiment, the present invention relates to acombination of any component A mentioned herein with any component Bmentioned herein, optionally with any component C mentioned herein.

In one embodiment component A of the combination is the compound used inthe experimental section and Component B is radium-223 dichloride(Ra²²³Cl₂) as being used in the experimental section.

In a particular embodiment, the present invention relates to acombination of a component A with a component B, optionally with acomponent C, as mentioned in the Examples Section herein.

Further, the present invention relates to:

a kit comprising:

-   -   a combination of:

-   component A: one or more PI3K-kinase inhibitors, or a    physiologically acceptable salt, solvate, hydrate or stereoisomer    thereof;

-   component B: a suitable pharmaceutically acceptable salt of the    alkaline-earth radionuclide radium-223 or a solvate or a hydrate    thereof; and, optionally,

-   component C: one or more further pharmaceutical agents;    in which optionally either or both of said components A and B in any    of the above-mentioned combinations are in the form of a    pharmaceutical formulation which is ready for use to be administered    simultaneously, concurrently, separately or sequentially.

The term “component C” being at least one pharmaceutical agent includesthe effective compound itself as well as its pharmaceutically acceptablesalts, solvates, hydrates or stereoisomers as well as any composition orpharmaceutical formulation comprising such effective compound or itspharmaceutically acceptable salts, solvates, hydrates or stereoisomers.A list of such readily available agents is being provided further below.

The components may be administered independently of one another by theoral, intravenous, topical, local installations, intraperitoneal ornasal route.

Component A is administered intravenously, intraperitoneally, preferablyit is administered orally.

Component B preferably is administered by the intravenous route.

Component C being administered as the case may be.

The term “pharmaceutically acceptable” is used synonymously to the term“physiologically acceptable”.

The term “pharmaceutically or physiologically acceptable salt” ofcomponent A refers to a relatively non-toxic, inorganic or organic acidaddition salt of a compound of the present invention. For example, seeS. M. Berge, et al. “Pharmaceutical Salts,” J. Pharm. Sci. 1977, 66,1-19. Pharmaceutically acceptable salts include those obtained byreacting the main compound, functioning as a base, with an inorganic ororganic acid to form a salt, for example, salts of hydrochloric acid,sulfuric acid, phosphoric acid, methane sulfonic acid, camphor sulfonicacid, oxalic acid, maleic acid, succinic acid and citric acid.Pharmaceutically acceptable salts also include those in which the maincompound functions as an acid and is reacted with an appropriate base toform, e.g., sodium, potassium, calcium, magnesium, ammonium, and chorinesalts. Those skilled in the art will further recognize that acidaddition salts of the claimed compounds may be prepared by reaction ofthe compounds with the appropriate inorganic or organic acid via any ofa number of known methods. Alternatively, alkali and alkaline earthmetal salts of acidic compounds of the invention are prepared byreacting the compounds of the invention with the appropriate base via avariety of known methods.

Representative salts of a component A of this invention include theconventional non-toxic salts and the quaternary ammonium salts which areformed, for example, from inorganic or organic acids or bases by meanswell known in the art. For example, such acid addition salts includeacetate, adipate, alginate, ascorbate, aspartate, benzoate,benzenesulfonate, bisulfate, butyrate, citrate, camphorate,camphorsulfonate, cinnamate, cyclopentanepropionate, digluconate,dodecylsulfate, ethanesulfonate, fumarate, glucoheptanoate,glycerophosphate, hemisulfate, heptanoate, hexanoate, chloride, bromide,iodide, 2-hydroxyethanesulfonate, itaconate, lactate, maleate,mandelate, methanesulfonate, 2-naphthalenesulfonate, nicotinate,nitrate, oxalate, pamoate, pectinate, persulfate, 3-phenylpropionate,picrate, pivalate, propionate, succinate, sulfonate, sulfate, tartrate,thiocyanate, tosylate, and undecanoate.

Base salts include alkali metal salts such as potassium and sodiumsalts, alkaline earth metal salts such as calcium and magnesium salts,and ammonium salts with organic bases such as dicyclohexylamine andN-methyl-D-glucamine. Additionally, basic nitrogen containing groups maybe quaternized with such agents as lower alkyl halides such as methyl,ethyl, propyl, or butyl chlorides, bromides and iodides; dialkylsulfates like dimethyl, diethyl, dibutyl sulfate, or diamyl sulfates,long chain halides such as decyl, lauryl, myristyl and strearylchlorides, bromides and iodides, aralkyl halides like benzyl andphenethyl bromides and others.

A solvate for the purpose of this invention is a complex of a solventand a compound of the invention in the solid state. Exemplary solvateswould include, but are not limited to, complexes of a compound of theinvention with ethanol or methanol. Hydrates are a specific form ofsolvate wherein the solvent is water.

Components of this invention can be tableted with conventional tabletbases such as lactose, sucrose and cornstarch in combination withbinders such as acacia, corn starch or gelatin, disintegrating agentsintended to assist the break-up and dissolution of the tablet followingadministration such as potato starch, alginic acid, corn starch, andguar gum, gum tragacanth, acacia, lubricants intended to improve theflow of tablet granulation and to prevent the adhesion of tabletmaterial to the surfaces of the tablet dies and punches, for exampletalc, stearic acid, or magnesium, calcium or zinc stearate, dyes,coloring agents, and flavoring agents such as peppermint, oil ofwintergreen, or cherry flavoring, intended to enhance the aestheticqualities of the tablets and make them more acceptable to the patient.Suitable excipients for use in oral liquid dosage forms includedicalcium phosphate and diluents such as water and alcohols, forexample, ethanol, benzyl alcohol, and polyethylene alcohols, either withor without the addition of a pharmaceutically acceptable surfactant,suspending agent or emulsifying agent. Various other materials may bepresent as coatings or to otherwise modify the physical form of thedosage unit. For instance tablets, pills or capsules may be coated withshellac, sugar or both.

Dispersible powders and granules are suitable for the preparation of anaqueous suspension. They provide the active ingredient in admixture witha dispersing or wetting agent, a suspending agent and one or morepreservatives. Suitable dispersing or wetting agents and suspendingagents are exemplified by those already mentioned above. Additionalexcipients, for example those sweetening, flavoring and coloring agentsdescribed above, may also be present.

Components of this invention can also be in the form of oil-in-wateremulsions. The oily phase may be a vegetable oil such as liquid paraffinor a mixture of vegetable oils. Suitable emulsifying agents may be (1)naturally occurring gums such as gum acacia and gum tragacanth, (2)naturally occurring phosphatides such as soy bean and lecithin, (3)esters or partial esters derived form fatty acids and hexitolanhydrides, for example, sorbitan monooleate, (4) condensation productsof said partial esters with ethylene oxide, for example, polyoxyethylenesorbitan monooleate. The emulsions may also contain sweetening andflavoring agents.

Oily suspensions can be formulated by suspending the active ingredientin a vegetable oil such as, for example, arachis oil, olive oil, sesameoil or coconut oil, or in a mineral oil such as liquid paraffin. Theoily suspensions may contain a thickening agent such as, for example,beeswax, hard paraffin, or cetyl alcohol. The suspensions may alsocontain one or more preservatives, for example, ethyl or n-propylp-hydroxybenzoate; one or more coloring agents; one or more flavoringagents; and one or more sweetening agents such as sucrose or saccharin.

Syrups and elixirs can be formulated with sweetening agents such as, forexample, glycerol, propylene glycol, sorbitol or sucrose. Suchformulations may also contain a demulcent, and preservative, such asmethyl and propyl parabens and flavoring and coloring agents.

Components of this invention can also be administered parenterally, thatis, subcutaneously, intravenously, intraocularly, intrasynovially,intramuscularly, or interperitoneally, as injectable dosages of thecompound in preferably a physiologically acceptable diluent with apharmaceutical carrier which can be a sterile liquid or mixture ofliquids such as water, saline, aqueous dextrose and related sugarsolutions, an alcohol such as ethanol, isopropanol, or hexadecylalcohol, glycols such as propylene glycol or polyethylene glycol,glycerol ketals such as 2,2-dimethyl-1,1-dioxolane-4-methanol, etherssuch as poly(ethylene glycol) 400, an oil, a fatty acid, a fatty acidester or, a fatty acid glyceride, or an acetylated fatty acid glyceride,with or without the addition of a pharmaceutically acceptable surfactantsuch as a soap or a detergent, suspending agent such as pectin,carbomers, methycellulose, hydroxypropylmethylcellulose, orcarboxymethylcellulose, or emulsifying agent and other pharmaceuticaladjuvants.

Illustrative of oils which can be used in the parenteral formulations ofthis invention are those of petroleum, animal, vegetable, or syntheticorigin, for example, peanut oil, soybean oil, sesame oil, cottonseedoil, corn oil, olive oil, petrolatum and mineral oil. Suitable fattyacids include oleic acid, stearic acid, isostearic acid and myristicacid. Suitable fatty acid esters are, for example, ethyl oleate andisopropyl myristate. Suitable soaps include fatty acid alkali metal,ammonium, and triethanolamine salts and suitable detergents includecationic detergents, for example dimethyl dialkyl ammonium halides,alkyl pyridinium halides, and alkylamine acetates; anionic detergents,for example, alkyl, aryl, and olefin sulfonates, alkyl, olefin, ether,and monoglyceride sulfates, and sulfosuccinates; non-ionic detergents,for example, fatty amine oxides, fatty acid alkanolamides, andpoly(oxyethylene-oxypropylene)s or ethylene oxide or propylene oxidecopolymers; and amphoteric detergents, for example,alkyl-beta-aminopropionates, and 2-alkylimidazoline quarternary ammoniumsalts, as well as mixtures.

The parenteral compositions of this invention will typically containfrom about 0.5% to about 25% by weight of the active ingredient insolution. Preservatives and buffers may also be used advantageously. Inorder to minimize or eliminate irritation at the site of injection, suchcompositions may contain a non-ionic surfactant having ahydrophile-lipophile balance (HLB) preferably of from about 12 to about17. The quantity of surfactant in such formulation preferably rangesfrom about 5% to about 15% by weight. The surfactant can be a singlecomponent having the above HLB or can be a mixture of two or morecomponents having the desired HLB.

Illustrative of surfactants used in parenteral formulations are theclass of polyethylene sorbitan fatty acid esters, for example, sorbitanmonooleate and the high molecular weight adducts of ethylene oxide witha hydrophobic base, formed by the condensation of propylene oxide withpropylene glycol.

The pharmaceutical compositions can be in the form of sterile injectableaqueous suspensions. Such suspensions may be formulated according toknown methods using suitable dispersing or wetting agents and suspendingagents such as, for example, sodium carboxymethylcellulose,methylcellulose, hydroxypropylmethyl-cellulose, sodium alginate,polyvinylpyrrolidone, gum tragacanth and gum acacia; dispersing orwetting agents which may be a naturally occurring phosphatide such aslecithin, a condensation product of an alkylene oxide with a fatty acid,for example, polyoxyethylene stearate, a condensation product ofethylene oxide with a long chain aliphatic alcohol, for example,heptadeca-ethyleneoxycetanol, a condensation product of ethylene oxidewith a partial ester derived form a fatty acid and a hexitol such aspolyoxyethylene sorbitol monooleate, or a condensation product of anethylene oxide with a partial ester derived from a fatty acid and ahexitol anhydride, for example polyoxyethylene sorbitan monooleate.

The sterile injectable preparation can also be a sterile injectablesolution or suspension in a non-toxic parenterally acceptable diluent orsolvent. Diluents and solvents that may be employed are, for example,water, Ringer's solution, isotonic sodium chloride solutions andisotonic glucose solutions. In addition, sterile fixed oils areconventionally employed as solvents or suspending media. For thispurpose, any bland, fixed oil may be employed including synthetic mono-or diglycerides. In addition, fatty acids such as oleic acid can be usedin the preparation of injectables.

Components of the invention can also be administered in the form ofsuppositories for rectal administration of the drug. These componentscan be prepared by mixing the drug with a suitable non-irritationexcipient which is solid at ordinary temperatures but liquid at therectal temperature and will therefore melt in the rectum to release thedrug. Such materials are, for example, cocoa butter and polyethyleneglycol.

Another formulation employed in the methods of the present inventionemploys transdermal delivery devices (“patches”). Such transdermalpatches may be used to provide continuous or discontinuous infusion ofthe compounds of the present invention in controlled amounts. Theconstruction and use of transdermal patches for the delivery ofpharmaceutical agents is well known in the art (see, e.g., U.S. Pat. No.5,023,252, issued Jun. 11, 1991, incorporated herein by reference). Suchpatches may be constructed for continuous, pulsatile, or on demanddelivery of pharmaceutical agents.

Controlled release formulations for parenteral administration includeliposomal, polymeric microsphere and polymeric gel formulations that areknown in the art.

It can be desirable or necessary to introduce a component of the presentinvention to the patient via a mechanical delivery device. Theconstruction and use of mechanical delivery devices for the delivery ofpharmaceutical agents is well known in the art. Direct techniques for,for example, administering a drug directly to the brain usually involveplacement of a drug delivery catheter into the patient's ventricularsystem to bypass the blood-brain barrier. One such implantable deliverysystem, used for the transport of agents to specific anatomical regionsof the body, is described in U.S. Pat. No. 5,011,472, issued Apr. 30,1991.

The compositions of the invention can also contain other conventionalpharmaceutically acceptable compounding ingredients, generally referredto as carriers or diluents, as necessary or desired. Conventionalprocedures for preparing such compositions in appropriate dosage formscan be utilized. Such ingredients and procedures include those describedin the following references, each of which is incorporated herein byreference: Powell, M. F. et al, “Compendium of Excipients for ParenteralFormulations” PDA Journal of Pharmaceutical Science & Technology 1998,52(5), 238-311; Strickley, R. G “Parenteral Formulations of SmallMolecule Therapeutics Marketed in the United States (1999)—Part-1” PDAJournal of Pharmaceutical Science & Technology 1999, 53(6), 324-349; andNema, S. et al, “Excipients and Their Use in Injectable Products” PDAJournal of Pharmaceutical Science & Technology 1997, 51(4), 166-171.

Commonly used pharmaceutical ingredients that can be used as appropriateto formulate the composition for its intended route of administrationinclude:

acidifying agents (examples include but are not limited to acetic acid,citric acid, fumaric acid, hydrochloric acid, nitric acid);

alkalinizing agents (examples include but are not limited to ammoniasolution, ammonium carbonate, diethanolamine, monoethanolamine,potassium hydroxide, sodium borate, sodium carbonate, sodium hydroxide,triethanolamine, trolamine);

adsorbents (examples include but are not limited to powdered celluloseand activated charcoal);

aerosol propellants (examples include but are not limited to carbondioxide, CCl₂F₂, F₂ClC—CClF₂ and CClF₃)

air displacement agents (examples include but are not limited tonitrogen and argon);

antifungal preservatives (examples include but are not limited tobenzoic acid, butylparaben, ethylparaben, methylparaben, propylparaben,sodium benzoate);

antimicrobial preservatives (examples include but are not limited tobenzalkonium chloride, benzethonium chloride, benzyl alcohol,cetylpyridinium chloride, chlorobutanol, phenol, phenylethyl alcohol,phenylmercuric nitrate and thimerosal);

antioxidants (examples include but are not limited to ascorbic acid,ascorbyl palmitate, butylated hydroxyanisole, butylated hydroxytoluene,hypophosphorus acid, monothioglycerol, propyl gallate, sodium ascorbate,sodium bisulfite, sodium formaldehyde sulfoxylate, sodiummetabisulfite);binding materials (examples include but are not limited to blockpolymers, natural and synthetic rubber, polyacrylates, polyurethanes,silicones, polysiloxanes and styrene-butadiene copolymers);buffering agents (examples include but are not limited to potassiummetaphosphate, dipotassium phosphate, sodium acetate, sodium citrateanhydrous and sodium citrate dihydrate)carrying agents (examples include but are not limited to acacia syrup,aromatic syrup, aromatic elixir, cherry syrup, cocoa syrup, orangesyrup, syrup, corn oil, mineral oil, peanut oil, sesame oil,bacteriostatic sodium chloride injection and bacteriostatic water forinjection)chelating agents (examples include but are not limited to edetatedisodium and edetic acid)colorants (examples include but are not limited to FD&C Red No. 3, FD&CRed No. 20, FD&C Yellow No. 6, FD&C Blue No. 2, D&C Green No. 5, D&COrange No. 5, D&C Red No. 8, caramel and ferric oxide red);clarifying agents (examples include but are not limited to bentonite);emulsifying agents (examples include but are not limited to acacia,cetomacrogol, cetyl alcohol, glyceryl monostearate, lecithin, sorbitanmonooleate, polyoxyethylene 50 monostearate);encapsulating agents (examples include but are not limited to gelatinand cellulose acetate phthalate)flavorants (examples include but are not limited to anise oil, cinnamonoil, cocoa, menthol, orange oil, peppermint oil and vanillin);humectants (examples include but are not limited to glycerol, propyleneglycol and sorbitol);levigating agents (examples include but are not limited to mineral oiland glycerin);oils (examples include but are not limited to arachis oil, mineral oil,olive oil, peanut oil, sesame oil and vegetable oil);ointment bases (examples include but are not limited to lanolin,hydrophilic ointment, polyethylene glycol ointment, petrolatum,hydrophilic petrolatum, white ointment, yellow ointment, and rose waterointment);penetration enhancers (transdermal delivery) (examples include but arenot limited to monohydroxy or polyhydroxy alcohols, mono- or polyvalentalcohols, saturated or unsaturated fatty alcohols, saturated orunsaturated fatty esters, saturated or unsaturated dicarboxylic acids,essential oils, phosphatidyl derivatives, cephalin, terpenes, amides,ethers, ketones and ureas)plasticizers (examples include but are not limited to diethyl phthalateand glycerol);solvents (examples include but are not limited to ethanol, corn oil,cottonseed oil, glycerol, isopropanol, mineral oil, oleic acid, peanutoil, purified water, water for injection, sterile water for injectionand sterile water for irrigation);stiffening agents (examples include but are not limited to cetylalcohol, cetyl esters wax, microcrystalline wax, paraffin, stearylalcohol, white wax and yellow wax);suppository bases (examples include but are not limited to cocoa butterand polyethylene glycols (mixtures));surfactants (examples include but are not limited to benzalkoniumchloride, nonoxynol 10, oxtoxynol 9, polysorbate 80, sodium laurylsulfate and sorbitan mono-palmitate);suspending agents (examples include but are not limited to agar,bentonite, carbomers, carboxymethylcellulose sodium, hydroxyethylcellulose, hydroxypropyl cellulose, hydroxypropyl methylcellulose,kaolin, methylcellulose, tragacanth and veegum);sweetening agents (examples include but are not limited to aspartame,dextrose, glycerol, mannitol, propylene glycol, saccharin sodium,sorbitol and sucrose);tablet anti-adherents (examples include but are not limited to magnesiumstearate and talc);tablet binders (examples include but are not limited to acacia, alginicacid, carboxymethylcellulose sodium, compressible sugar, ethylcellulose,gelatin, liquid glucose, methylcellulose, non-crosslinked polyvinylpyrrolidone, and pregelatinized starch);tablet and capsule diluents (examples include but are not limited todibasic calcium phosphate, kaolin, lactose, mannitol, microcrystallinecellulose, powdered cellulose, precipitated calcium carbonate, sodiumcarbonate, sodium phosphate, sorbitol and starch);tablet coating agents (examples include but are not limited to liquidglucose, hydroxyethyl cellulose, hydroxypropyl cellulose, hydroxypropylmethylcellulose, methylcellulose, ethylcellulose, cellulose acetatephthalate and shellac);tablet direct compression excipients (examples include but are notlimited to dibasic calcium phosphate);tablet disintegrants (examples include but are not limited to alginicacid, carboxymethylcellulose calcium, microcrystalline cellulose,polacrillin potassium, cross-linked polyvinylpyrrolidone, sodiumalginate, sodium starch glycollate and starch);tablet glidants (examples include but are not limited to colloidalsilica, corn starch and talc);tablet lubricants (examples include but are not limited to calciumstearate, magnesium stearate, mineral oil, stearic acid and zincstearate);tablet/capsule opaquants (examples include but are not limited totitanium dioxide);tablet polishing agents (examples include but are not limited to carnubawax and white wax);thickening agents (examples include but are not limited to beeswax,cetyl alcohol and paraffin);tonicity agents (examples include but are not limited to dextrose andsodium chloride);viscosity increasing agents (examples include but are not limited toalginic acid, bentonite, carbomers, carboxymethylcellulose sodium,methylcellulose, polyvinyl pyrrolidone, sodium alginate and tragacanth);andwetting agents (examples include but are not limited toheptadecaethylene oxycetanol, lecithins, sorbitol monooleate,polyoxyethylene sorbitol monooleate, and polyoxyethylene stearate).

Pharmaceutical compositions according to the present invention can beillustrated as follows:

Sterile IV Solution:

A 5 mg/mL solution of the desired compound of this invention can be madeusing sterile, injectable water, and the pH is adjusted if necessary.The solution is diluted for administration to 1-2 mg/mL with sterile 5%dextrose and is administered as an IV infusion over about 60 minutes.

Lyophilized Powder for IV Administration:

A sterile preparation can be prepared with (i) 100-1000 mg of thedesired compound of this invention as a lypholized powder, (ii) 32-327mg/mL sodium citrate, and (iii) 300-3000 mg Dextran 40. The formulationis reconstituted with sterile, injectable saline or dextrose 5% to aconcentration of 10 to 20 mg/mL, which is further diluted with saline ordextrose 5% to 0.2-0.4 mg/mL, and is administered either IV bolus or byIV infusion over 15-60 minutes.

Intramuscular Suspension:

The following solution or suspension can be prepared, for intramuscularinjection:

50 mg/mL of the desired, water-insoluble compound of this invention

5 mg/mL sodium carboxymethylcellulose

4 mg/mL TWEEN 80

9 mg/mL sodium chloride

9 mg/mL benzyl alcohol

Hard Shell Capsules:

A large number of unit capsules are prepared by filling standardtwo-piece hard galantine capsules each with 100 mg of powdered activeingredient, 150 mg of lactose, 50 mg of cellulose and 6 mg of magnesiumstearate.

Soft Gelatin Capsules:

A mixture of active ingredient in a digestible oil such as soybean oil,cottonseed oil or olive oil is prepared and injected by means of apositive displacement pump into molten gelatin to form soft gelatincapsules containing 100 mg of the active ingredient. The capsules arewashed and dried. The active ingredient can be dissolved in a mixture ofpolyethylene glycol, glycerin and sorbitol to prepare a water misciblemedicine mix.

Tablets:

A large number of tablets are prepared by conventional procedures sothat the dosage unit is 100 mg of active ingredient, 0.2 mg. ofcolloidal silicon dioxide, 5 mg of magnesium stearate, 275 mg ofmicrocrystalline cellulose, 11 mg. of starch, and 98.8 mg of lactose.Appropriate aqueous and non-aqueous coatings may be applied to increasepalatability, improve elegance and stability or delay absorption.

Immediate Release Tablets/Capsules:

These are solid oral dosage forms made by conventional and novelprocesses. These units are taken orally without water for immediatedissolution and delivery of the medication. The active ingredient ismixed in a liquid containing ingredient such as sugar, gelatin, pectinand sweeteners. These liquids are solidified into solid tablets orcaplets by freeze drying and solid state extraction techniques. The drugcompounds may be compressed with viscoelastic and thermoelastic sugarsand polymers or effervescent components to produce porous matricesintended for immediate release, without the need of water.

Commercial Utility

Component A

The compounds of formula (A) and (I) and the stereoisomers thereofaccording to the combination as referred to above are components A. Thecompounds according to the combination have valuable pharmaceuticalproperties, which make them commercially utilizable. In particular, theyinhibit the PI3K/AKT pathway and exhibit cellular activity. They areexpected to be commercially applicable in the therapy of diseases (e.g.diseases dependent on overactivated PI3K/AKT). An abnormal activation ofthe PI3K/AKT pathway is an essential step towards the initiation andmaintenance of human tumors and thus its inhibition, for example withPI3K inhibitors, is understood to be a valid approach for treatment ofhuman tumors. For a recent review see Garcia-Echeverria et al (Oncogene,2008, 27, 551-5526.

Component B

Due to the mechanism as discussed in the introductory section componentB is especially suitable to have effects on tumor diseases, especiallythose developing metastases in bones.

Combination

The combinations of the present invention thus can be used for thetreatment or prophylaxis of diseases of uncontrolled cell growth,proliferation and/or survival, inappropriate cellular immune responses,or inappropriate cellular inflammatory responses, or diseases which areaccompanied with uncontrolled cell growth, proliferation and/orsurvival, inappropriate cellular immune responses, or inappropriatecellular inflammatory responses, particularly in which the uncontrolledcell growth, proliferation and/or survival, inappropriate cellularimmune responses, or inappropriate cellular inflammatory responses, suchas, for example, haematological tumours and/or metastases thereof, solidtumours, and/or metastases thereof, e.g. leukaemias, multiple myelomathereof and myelodysplastic syndrome, malignant lymphomas, breasttumours including and bone metastases thereof, tumours of the thoraxincluding non-small cell and small cell lung tumours and bone metastasesthereof, gastrointestinal tumours, endocrine tumours, mammary and othergynaecological tumours and bone metastases thereof, urological tumoursincluding renal, bladder and prostate tumours, skin tumours, andsarcomas, and/or metastases thereof.

One embodiment relates to the use of a combination according to any oneof claims 1 to 12 for the preparation of a medicament for the treatmentor prophylaxis of a cancer, particularly breast cancer, prostate cancer,multiple myeloma, hepatocyte carcinoma, lung cancer, in particularnon-small cell lung carcinoma, colorectal cancer, melanoma, pancreaticcancer and/or metastases thereof.

In one embodiment the invention relates to combinations comprisingcomponent A or a pharmaceutically acceptable salt thereof and ComponentB being a pharmaceutically acceptable salt of the alkaline earthradionuclide radium-223 for use in the treatment of cancer indicationsparticularly for such cancer type which is known to form metastases inbone.

Such cancer types are e.g. breast, prostate, lung, multiple myeloma,kidney or thyroid cancer.

Another embodiment relates to the use of a combination according to thepresent invention for the preparation of a medicament for the treatmentor prophylaxis of breast cancer, prostate cancer, multiple myeloma,non-small cell lung cancer and/or metastases thereof, especially whereinthe metastases are bone metastases.

In one embodiment the invention relates to a method of treatment orprophylaxis of a cancer, particularly breast cancer, prostate cancer,multiple myeloma, hepatocyte carcinoma, lung cancer, in particularnon-small cell lung carcinoma, colorectal cancer, melanoma, orpancreatic cancer, in a subject, comprising administering to saidsubject a therapeutically effective amount of a combination according toany one of claims 1 to 12.

In another embodiment the invention relates to a method of treatment orprophylaxis of a cancer, particularly breast cancer, prostate cancer,multiple myeloma, hepatocyte carcinoma, lung cancer, in particularnon-small cell lung carcinoma, colorectal cancer, melanoma, orpancreatic cancer, in a subject, comprising administering to saidsubject a therapeutically effective amount of a combination according toany one of claims 1 to 12.

In another embodiment the invention relates to a method of treatment orprophylaxis of a cancer, particularly breast cancer, prostate cancer,multiple myeloma, hepatocyte carcinoma, lung cancer, in particularnon-small cell lung carcinoma, colorectal cancer, melanoma, orpancreatic cancer and/or metastases thereof in a subject, comprisingadministering to said subject a therapeutically effective amount of acombination according to any one of claims 1 to 12.

Preferred uses of the combinations of the invention are the treatment ofmultiple myeloma, lung, breast and prostate cancer, especiallycastration-resistant prostate cancer (CRPC), and bone metastases.

One preferred embodiment is the use of the combinations of the inventionfor the treatment of prostate cancer, especially castration-resistantprostate cancer (CRPC) and bone metastases.

One preferred embodiment is the use of the combinations of the inventionfor the treatment of breast cancer and bone metastases.

The term “inappropriate” within the context of the present invention, inparticular in the context of “inappropriate cellular immune responses,or inappropriate cellular inflammatory responses”, as used herein, is tobe understood as preferably meaning a response which is less than, orgreater than normal, and which is associated with, responsible for, orresults in, the pathology of said diseases.

Combinations of the present invention might be utilized to inhibit,block, reduce, decrease, etc., cell proliferation and/or cell division,and/or produce apoptosis.

This invention includes a method comprising administering to a mammal inneed thereof, including a human, an amount of a component A and anamount of component B of this invention, or a pharmaceuticallyacceptable salt, isomer, polymorph, metabolite, hydrate, solvate orester thereof; etc. which is effective to treat the disorder.

Hyper-proliferative disorders include but are not limited, e.g.,psoriasis, keloids, and other hyperplasias affecting the skin, benignprostate hyperplasia (BPH), as well as malignant neoplasia. Examples ofmalignant neoplasia treatable with the compounds according to thepresent invention include solid and hematological tumors. Solid tumorscan be exemplified by tumors of the breast, bladder, bone, brain,central and peripheral nervous system, colon, anum, endocrine glands(e.g. thyroid and adrenal cortex), esophagus, endometrium, germ cells,head and neck, kidney, liver, lung, larynx and hypopharynx,mesothelioma, ovary, pancreas, prostate, rectum, renal, small intestine,soft tissue, testis, stomach, skin, ureter, vagina and vulva. Malignantneoplasias include inherited cancers exemplified by Retinoblastoma andWilms tumor. In addition, malignant neoplasias include primary tumors insaid organs and corresponding secondary tumors in distant organs (“tumormetastases”). Hematological tumors can be exemplified by aggressive andindolent forms of leukemia and lymphoma, namely non-Hodgkins disease,chronic and acute myeloid leukemia (CML/AML), acute lymphoblasticleukemia (ALL), Hodgkins disease, multiple myeloma and T-cell lymphoma.Also included are myelodysplastic syndrome, plasma cell neoplasia,paraneoplastic syndromes, and cancers of unknown primary site as well asAIDS related malignancies.

Examples of breast cancer include, but are not limited to invasiveductal carcinoma, invasive lobular carcinoma, ductal carcinoma in situ,and lobular carcinoma in situ, particularly with bone metastases.

Examples of cancers of the respiratory tract include, but are notlimited to small-cell and non-small-cell lung carcinoma, as well asbronchial adenoma and pleuropulmonary blastoma.

Examples of brain cancers include, but are not limited to brain stem andhypophtalmic glioma, cerebellar and cerebral astrocytoma,medulloblastoma, ependymoma, as well as neuroectodermal and pinealtumor.

Tumors of the male reproductive organs include, but are not limited toprostate and testicular cancer. Tumors of the female reproductive organsinclude, but are not limited to endometrial, cervical, ovarian, vaginal,and vulvar cancer, as well as sarcoma of the uterus.

Tumors of the digestive tract include, but are not limited to anal,colon, colorectal, esophageal, gallbladder, gastric, pancreatic, rectal,small-intestine, and salivary gland cancers.

Tumors of the urinary tract include, but are not limited to bladder,penile, kidney, renal pelvis, ureter, urethral and human papillary renalcancers.

Eye cancers include, but are not limited to intraocular melanoma andretinoblastoma.

Examples of liver cancers include, but are not limited to hepatocellularcarcinoma (liver cell carcinomas with or without fibrolamellar variant),cholangiocarcinoma (intrahepatic bile duct carcinoma), and mixedhepatocellular cholangiocarcinoma.

Skin cancers include, but are not limited to squamous cell carcinoma,Kaposi's sarcoma, malignant melanoma, Merkel cell skin cancer, andnon-melanoma skin cancer.

Head-and-neck cancers include, but are not limited to laryngeal,hypopharyngeal, nasopharyngeal, oropharyngeal cancer, lip and oralcavity cancer and squamous cell. Lymphomas include, but are not limitedto AIDS-related lymphoma, non-Hodgkin's lymphoma, cutaneous T-celllymphoma, Burkitt lymphoma, Hodgkin's disease, and lymphoma of thecentral nervous system.

Sarcomas include, but are not limited to sarcoma of the soft tissue,osteosarcoma, malignant fibrous histiocytoma, lymphosarcoma, andrhabdomyosarcoma.

Leukemias include, but are not limited to acute myeloid leukemia, acutelymphoblastic leukemia, chronic lymphocytic leukemia, chronicmyelogenous leukemia, and hairy cell leukemia.

These disorders have been well characterized in humans, but also existwith a similar etiology in other mammals, and can be treated byadministering pharmaceutical compositions of the present invention.

The term “treating” or “treatment” as stated throughout this document isused conventionally, e.g., the management or care of a subject for thepurpose of combating, alleviating, reducing, relieving, improving thecondition of, etc., of a disease or disorder, such as a carcinoma.

Combinations of the present invention might also be used for treatingdisorders and diseases associated with excessive and/or abnormalangiogenesis.

Inappropriate and ectopic expression of angiogenesis can be deleteriousto an organism. A number of pathological conditions are associated withthe growth of extraneous blood vessels. These include, e.g., diabeticretinopathy, ischemic retinal-vein occlusion, and retinopathy ofprematurity [Aiello et al. New Engl. J. Med. 1994, 331, 1480; Peer etal. Lab. Invest. 1995, 72, 638], age-related macular degeneration [AMD;see, Lopez et al. Invest. Opththalmol. Vis. Sci. 1996, 37, 855],neovascular glaucoma, psoriasis, retrolental fibroplasias, angiofibroma,inflammation, rheumatoid arthritis (RA), restenosis, in-stentrestenosis, vascular graft restenosis, etc. In addition, the increasedblood supply associated with cancerous and neoplastic tissue, encouragesgrowth, leading to rapid tumor enlargement and metastasis. Moreover, thegrowth of new blood and lymph vessels in a tumor provides an escaperoute for renegade cells, encouraging metastasis and the consequencespread of the cancer. Thus, combinations of the present invention can beutilized to treat and/or prevent any of the aforementioned angiogenesisdisorders, e.g., by inhibiting and/or reducing blood vessel formation;by inhibiting, blocking, reducing, decreasing, etc. endothelial cellproliferation or other types involved in angiogenesis, as well ascausing cell death or apoptosis of such cell types.

Dose and Administration

Component A

Based upon standard laboratory techniques known to evaluate compoundsuseful for the treatment of hyper-proliferative disorders and angiogenicdisorders, by standard toxicity tests and by standard pharmacologicalassays for the determination of treatment of the conditions identifiedabove in mammals, and by comparison of these results with the results ofknown medicaments that are used to treat these conditions, the effectivedosage of the compounds of this invention can readily be determined fortreatment of each desired indication. The amount of the activeingredients to be administered in the treatment of one of theseconditions can vary widely according to such considerations as theparticular component And dosage unit employed, the mode ofadministration, the period of treatment, the age and sex of the patienttreated, and the nature and extent of the condition treated.

The total amount of the active ingredients to be administered willgenerally range from about 0.001 mg/kg to about 200 mg/kg body weightper day, and preferably from about 0.01 mg/kg to about 20 mg/kg bodyweight per day. Clinically useful dosing schedules of a compound willrange from one to three times a day dosing to once every four weeksdosing. In addition, “drug holidays” in which a patient is not dosedwith a drug for a certain period of time, may be beneficial to theoverall balance between pharmacological effect and tolerability. A unitdosage may contain from about 0.5 mg to about 1500 mg of activeingredient, and can be administered one or more times per day or lessthan once a day. The average daily dosage for administration byinjection, including intravenous, intramuscular, subcutaneous andparenteral injections, and use of infusion techniques will preferably befrom 0.01 to 200 mg/kg of total body weight. The average daily rectaldosage regimen will preferably be from 0.01 to 200 mg/kg of total bodyweight. The average daily vaginal dosage regimen will preferably be from0.01 to 200 mg/kg of total body weight. The average daily topical dosageregimen will preferably be from 0.1 to 200 mg administered between oneto four times daily. The transdermal concentration will preferably bethat required to maintain a daily dose of from 0.01 to 200 mg/kg. Theaverage daily inhalation dosage regimen will preferably be from 0.01 to100 mg/kg of total body weight.

Component B

A preferred dosage regimen for radium-223 injection is 50 kBq per kgbody weight given at 4 week intervals, as a course consisting of 6injections. Single radium-223 doses up to 250 kBq per kg body weightwere evaluated in a phase I clinical trial. The observed adversereactions at this dose were diarrhea and reversible myelosuppression(including one case (⅕) of grade 3 neutropenia).

As an example, the aqueous radium-223 dichloride solution may besupplied in a single-dose 10 ml vial which contains a fill volume of 6ml. This product has a radioactivity concentration of radium-223 of1,000 kBq/mL (0.03 mCi/mL), corresponding to 0.53 ng/mL of radium atreference date.

Radium-223 is to be administered intravenously by qualified personnel asa slow bolus injection. An intravenous access line should be used foradministration of Radium-223. The line must be flushed with isotonicsaline before and after injection of Radium-223.

Of course the specific initial and continuing dosage regimen for eachpatient will vary according to the nature and severity of the conditionas determined by the attending diagnostician, the activity of thespecific compounds employed, the age and general condition of thepatient, time of administration, route of administration, rate ofexcretion of the drug, drug combinations, and the like. The desired modeof treatment and number of doses of a compound of the present inventionor a pharmaceutically acceptable salt or ester or composition thereofcan be ascertained by those skilled in the art using conventionaltreatment tests.

Combinations of the Present Invention

The combinations of the present invention can be used in particular intherapy and prevention, i.e. prophylaxis, of tumour growth andmetastases, especially in solid tumours of all indications and stageswith or without pre-treatment of the tumour growth, more especiallythose tumor types spreading into bones.

Methods of testing for a particular pharmacological or pharmaceuticalproperty are well known to persons skilled in the art.

The combinations of component A and component B of this invention can beadministered as the sole pharmaceutical agent or in combination with oneor more further pharmaceutical agents C where the resulting combinationof components A, B and C causes no unacceptable adverse effects. Forexample, the combinations of components A and B of this invention can becombined with component C, i.e. one or more further pharmaceuticalagents, such as known anti-angiogenesis, anti-hyper-proliferative,antiinflammatory, analgesic, immunoregulatory, diuretic, antiarrhytmic,anti-hypercholsterolemia, anti-dyslipidemia, anti-diabetic or antiviralagents, and the like, as well as with admixtures and combinationsthereof.

Component C, can be one or more pharmaceutical agents such as131I-chTNT, abarelix, abiraterone, aclarubicin, aldesleukin,alemtuzumab, alitretinoin, altretamine, aminoglutethimide, amrubicin,amsacrine, anastrozole, arglabin, arsenic trioxide, asparaginase,azacitidine, basiliximab, BAY 1143269, BAY 1000394, belotecan,bendamustine, bevacizumab, bexarotene, bicalutamide, bisantrene,bleomycin, bortezomib, buserelin, busulfan, cabazitaxel, calciumfolinate, calcium levofolinate, capecitabine, carboplatin, carmofur,carmustine, catumaxomab, celecoxib, celmoleukin, cetuximab,chlorambucil, chlormadinone, chlormethine, cisplatin, cladribine,clodronic acid, clofarabine, crisantaspase, cyclophosphamide,cyproterone, cytarabine, dacarbazine, dactinomycin, darbepoetin alfa,dasatinib, daunorubicin, decitabine, degarelix, denileukin diftitox,denosumab, deslorelin, dibrospidium chloride, docetaxel, doxifluridine,doxorubicin, doxorubicin+estrone, eculizumab, edrecolomab, elliptiniumacetate, eltrombopag, endostatin, enocitabine, epirubicin, epitiostanol,epoetin alfa, epoetin beta, eptaplatin, eribulin, erlotinib, estradiol,estramustine, etoposide, everolimus, exemestane, fadrozole, filgrastim,fludarabine, fluorouracil, flutamide, formestane, fotemustine,fulvestrant, gallium nitrate, ganirelix, gefitinib, gemcitabine,gemtuzumab, glutoxim, goserelin, histamine dihydrochloride, histrelin,hydroxycarbamide, I-125 seeds, ibandronic acid, ibritumomab tiuxetan,idarubicin, ifosfamide, imatinib, imiquimod, improsulfan, interferonalfa, interferon beta, interferon gamma, ipilimumab, irinotecan,ixabepilone, lanreotide, lapatinib, lenalidomide, lenograstim, lentinan,letrozole, leuprorelin, levamisole, lisuride, lobaplatin, lomustine,lonidamine, masoprocol, medroxyprogesterone, megestrol, melphalan,mepitiostane, mercaptopurine, methotrexate, methoxsalen, Methylaminolevulinate, methyltestosterone, mifamurtide, miltefosine,miriplatin, mitobronitol, mitoguazone, mitolactol, mitomycin, mitotane,mitoxantrone, nedaplatin, nelarabine, nilotinib, nilutamide,nimotuzumab, nimustine, nitracrine, ofatumumab, omeprazole, oprelvekin,oxaliplatin, p53 gene therapy, paclitaxel, palifermin, palladium-103seed, pamidronic acid, panitumumab, pazopanib, pegaspargase, PEG-epoetinbeta (methoxy PEG-epoetin beta), pegfilgrastim, peginterferon alfa-2b,pemetrexed, pentazocine, pentostatin, peplomycin, perfosfamide,picibanil, pirarubicin, plerixafor, plicamycin, poliglusam,polyestradiol phosphate, polysaccharide-K, porfimer sodium,pralatrexate, prednimustine, procarbazine, quinagolide, radium-223chloride, raloxifene, raltitrexed, ranimustine, razoxane, refametinib,regorafenib, risedronic acid, rituximab, romidepsin, romiplostim,sargramostim, sipuleucel-T, sizofiran, sobuzoxane, sodium glycididazole,sorafenib, streptozocin, sunitinib, talaporfin, tamibarotene, tamoxifen,tasonermin, teceleukin, tegafur, tegafur+gimeracil+oteracil, temoporfin,temozolomide, temsirolimus, teniposide, testosterone, tetrofosmin,thalidomide, thiotepa, thymalfasin, tioguanine, tocilizumab, topotecan,toremifene, tositumomab, trabectedin, trastuzumab, treosulfan,tretinoin, trilostane, triptorelin, trofosfamide, tryptophan, ubenimex,valrubicin, vandetanib, vapreotide, vemurafenib, vinblastine,vincristine, vindesine, vinflunine, vinorelbine, vorinostat, vorozole,yttrium-90 glass microspheres, zinostatin, zinostatin stimalamer,zoledronic acid, zorubicin. or combinations thereof.

Alternatively, said component C can be one or more furtherpharmaceutical agents selected from gemcitabine, paclitaxel, cisplatin,carboplatin, sodium butyrate, 5-FU, doxirubicin, tamoxifen, etoposide,trastumazab, gefitinib, intron A, rapamycin, 17-AAG, U0126, insulin, aninsulin derivative, a PPAR ligand, a sulfonylurea drug, an α-glucosidaseinhibitor, a biguanide, a PTP-1B inhibitor, a DPP-IV inhibitor, a11-beta-HSD inhibitor, GLP-1, a GLP-1 derivative, GIP, a GIP derivative,PACAP, a PACAP derivative, secretin or a secretin derivative.

Optional anti-hyper-proliferative agents which can be added as componentC to the combination of components A and B of the present inventioninclude but are not limited to compounds listed on the cancerchemotherapy drug regimens in the 11^(th) Edition of the Merck Index,(1996), which is hereby incorporated by reference, such as asparaginase,bleomycin, carboplatin, carmustine, chlorambucil, cisplatin, colaspase,cyclophosphamide, cytarabine, dacarbazine, dactinomycin, daunorubicin,doxorubicin (adriamycine), epirubicin, etoposide, 5-fluorouracil,hexamethylmelamine, hydroxyurea, ifosfamide, irinotecan, leucovorin,lomustine, mechlorethamine, 6-mercaptopurine, mesna, methotrexate,mitomycin C, mitoxantrone, prednisolone, prednisone, procarbazine,raloxifen, streptozocin, tamoxifen, thioguanine, topotecan, vinblastine,vincristine, and vindesine.

Other anti-hyper-proliferative agents suitable for use as component Cwith the combination of components A and B of the present inventioninclude but are not limited to those compounds acknowledged to be usedin the treatment of neoplastic diseases in Goodman and Gilman's ThePharmacological Basis of Therapeutics (Ninth Edition), editor Molinoffet al., publ. by McGraw-Hill, pages 1225-1287, (1996), which is herebyincorporated by reference, such as aminoglutethimide, L-asparaginase,azathioprine, 5-azacytidine cladribine, busulfan, diethylstilbestrol,2′,2′-difluorodeoxycytidine, docetaxel, erythrohydroxynonyl adenine,ethinyl estradiol, 5-fluorodeoxyuridine, 5-fluorodeoxyuridinemonophosphate, fludarabine phosphate, fluoxymesterone, flutamide,hydroxyprogesterone caproate, idarubicin, interferon,medroxyprogesterone acetate, megestrol acetate, melphalan, mitotane,paclitaxel (when component B is not itself paclitaxel), pentostatin,N-phosphonoacetyl-L-aspartate (PALA), plicamycin, semustine, teniposide,testosterone propionate, thiotepa, trimethylmelamine, uridine, andvinorelbine.

Other anti-hyper-proliferative agents suitable for use as component Cwith the combination of components A and B of the present inventioninclude but are not limited to other anti-cancer agents such asepothilone and its derivatives, irinotecan, raloxifen and topotecan.

Generally, the use of cytotoxic and/or cytostatic agents as component Cin combination with a combination of components A and B of the presentinvention will serve to:

-   (1) yield better efficacy in reducing the growth of a tumor and/or    metastasis or even eliminate the tumor and/or metastasis as compared    to administration of either agent alone,-   (2) provide for the administration of lesser amounts of the    administered chemotherapeutic agents,-   (3) provide for a chemotherapeutic treatment that is well tolerated    in the patient with fewer deleterious pharmacological complications    than observed with single agent chemotherapies and certain other    combined therapies,-   (4) provide for treating a broader spectrum of different cancer    types in mammals, especially humans,-   (5) provide for a higher response rate among treated patients,-   (6) provide for a longer survival time among treated patients    compared to standard chemotherapy treatments,-   (8) provide a longer time for tumor progression, and/or-   (9) yield efficacy and tolerability results at least as good as    those of the agents used alone, compared to known instances where    other cancer agent combinations produce antagonistic effects.

EXPERIMENTAL SECTION Examples Demonstrating the Synergistic Effect ofthe Combinations of Components A and B of the Present Invention

Component A:

In this Experimental section and in the Figures, the term “compound A”is an example of component A and is compound Example 13 of WO2008/070150 A1 as shown herein: it is2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide,of structure:

or a solvate, hydrate or stereoisomer thereof.

In this Experimental Section and in the Figures, the term “compound A”refers to2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamidedihydrochloride, of structure:

or a solvate, hydrate or stereoisomer thereof.

The synthesis of compound A′ is described in European patent applicationnumber EP 11 161 111.7, and in PCT application number PCT/EP2012/055600published under WO 2012/136553, both of which are hereby incorporatedherein in their entirety by reference.

Component B:

In this Experimental Section and in the Figures, the term “compound B”or “alpharadin” refers to radium-223 dichloride, the synthesis of whichis disclosed in WO2000/040275, which is incorporated herein in itsentirety by reference.

Examples Demonstrating the Synergistic Effect of the Combinations ofCompound A′ and Compound B of the Present Invention

The effects of PI3K inhibitor compound A′ and compound B in MCF-7 and4T1 breast cancer cells and PC-3 and LNCaP prostate cancer cells wereinvestigated in vitro as single agent and in combination. The effectswere studied by measuring cell proliferation and apoptosis. Compound Band the reference inhibitor doxorubicin were added at day 0. The PI3Kinhibitors were added at day −1 (24 hours before addition of Alpharadin)and at day 4. The following two concentrations of the inhibitors and twoconcentrations of radium 223 in 4 replicates were tested:

TABLE 1 Cell line Dose 1 Dose 2 Compound A′ MCF-7 50 nM 500 nM 4T1 100nM 1000 nM PC-3 50 nM 500 nM LNCaP 100 nM 1000 nM Compound B MCF-7 1600Bq/ml 800 Bq/ml 4T1 1600 Bq/ml 800 Bq/ml PC-3 1600 Bq/ml 800 Bq/ml LNCaP1600 Bq/ml 400 Bq/ml

The effects on cell proliferation were studied using a WST-1 basedproliferation assay and the effects on apoptosis were studied inparallel plates by measuring caspase 3/7 activity. The measurements wereperformed at 4 different time points, days 1, 2, 3 and 5. Caspaseactivity was normalized to the cell number obtained with the WST-1 assayin parallel plates.

The reference compound doxorubicin inhibited proliferation in all testedcell lines; MCF-7, 4T1, PC-3 and LNCaP, describing that the assays wereperformed successfully and the results obtained are reliable.

DESCRIPTION OF THE FIGURES

Compound A′ is a PI3K inhibitor as mentioned above.

TABLE 2 Pathway Cell line Description Derived from Mutation MCF-7 humanmammary gland pleural effusion PIK3CA adenocarcinoma 4T1 mouse mammarygland mammary tumor adenocarcinoma PC-3 human prostate bone PTEN-deladenocarcinoma LNCaP human prostate carcinoma left supraclavicularPTEN-del lymph node

FIG. 1/4

The effects of compound A′ and compound B on MCF-7 cell proliferation atdays 1-5 and apoptosis induction at day 2.

(A) The results are shown as Absorbance (450 nm) measured in the WST-1proliferation assay (MEAN). (B) The results are shown as Caspase3/7/WST-1 values (MEAN). The results of the control group doxorubicin(C), Compound A′ groups (D1=50 nM and D2=500 nM) and compound B groups(aD1=1600 Bq/ml and aD2=800 Bq/ml) were compared to the baseline group.(B) The effects of PI3K inhibitor compound A′ and Radium 223 on MCF-7cell caspase 3/7 activity at days 2. The results are shown as Caspase3/7/WST-1 values (MEAN+SEM). The results of the control group (C), PI3Kinhibitor groups (D1 and D2) or Radium 223 groups (aD1 and aD2), and thecorresponding combo groups (D1+aD1, D1+aD2, D2+aD1 and D2+aD2) aredepicted.

FIG. 2/4

The effects of compound A′ and compound B on 4T1 breast tumor cellproliferation at days 1-5.

The results are shown as Absorbance (450 nm) measured in the WST-1proliferation assay (MEAN). The results of the control group doxorubicin(C), compound A′ groups (D1=100 nM and D2=1000 nM) and compound B groups(aD1=1600 Bq/ml and aD2=800 Bq/ml) and the corresponding combo groups(D1+aD1, D1+aD2, D2+aD1 and D2+aD2) are depicted.

FIG. 3/4

The effects of compound A′ and compound B on PC3 prostate tumor cellproliferation at days 1-5.

The results are shown as Absorbance (450 nm) measured in the WST-1proliferation assay (MEAN). The results of the control group doxorubicin(C), compound A′ groups (D1=50 nM and D2=500 nM) and compound B groups(aD1=1600 Bq/ml and aD2=800 Bq/ml) and the corresponding combo groups(D1+aD1, D1+aD2, D2+aD1 and D2+aD2) are depicted.

FIG. 4/4

The effects of compound A′ and compound B on LNCaP prostate tumor cellproliferation at days 1-5 and apoptosis induction at day 2.

(A) The results are shown as Absorbance (450 nm) measured in the WST-1proliferation assay (MEAN). (B) The results are shown as Caspase3/7/WST-1 values (MEAN+SEM). The results of the control groupdoxorubicin (C), compound A′ groups (D1=100 nM and D2=1000 nM) andcompound B groups (aD1=1600 Bq/ml and aD2=800 Bq/ml) and thecorresponding combo groups (D1+aD1, D1+aD2, D2+aD1 and D2+aD2) aredepicted.

CONCLUSIONS

In MCF7, a breast cancer cell line with ER+ and activating PIK3CAmutation, compound B showed only slight inhibition of tumor cellproliferation, while compound A′ showed potent anti-proliferativeactivity. Combination of compound A′ and compound B further enhanced theanti-proliferative effect. Monotherapy of compound B could not inducetumor cell death, while compound A′ only at high dose can induceapoptosis and tumor killing effects were further enhanced by combinationwith compound B.

In 4T1, a triple negative metastatic breast cancer cell line, compound Band compound A′ at lower (100 nM) dose showed only moderate inhibitionof tumor cell proliferation, while compound B at higher dose showedstrong anti-proliferative effects. Combination of compound A′ andcompound B showed synergistic effects.

In PC3, a AR-negative CRPC cell line with loss-of-function of tumorsuppressor PTEN, compound B showed moderate inhibition of tumor cellproliferation and better anti-proliferactive activity was observed withcompound A′. All the combination groups showed synergistic effectscompared to the corresponding monotherapy groups.

In LNCaP, a AR-positive CRPC cell line with loss-of-function of tumorsuppressor PTEN, compound B was effective at high dose (1600 Bq/mL) andcompound A′ showed strong anti-proliferative effects. Combination ofcompound A′ and compound B at the low doses (100 nM and 800 Bq/mL)showed synergistic anti-proliferative effects. Although the synergisticanti-proliferative effects could not be demonstrated in the high dosecombination group due to potent single agent activity of compound A′,strong synergy was observed in caspase 3/7 apoptosis assay in both dosegroups.

Taken together, combination of compound A′ and compound B demonstrateddirect and synergistic anti-tumor activity in all 4 breast and prostatetumor cell lines tested, synergistic induction of tumor cell death wasalso observed in hormone receptor positive MCF7 (ER+) breast cancer andLNCaP (AR+) prostate tumor cell lines.

In summary, our data indicate synergistic effects of the PI3K inhibitorcompound A′ and compound B in inhibiting tumor cell proliferation andsurvival, and warrant further clinical evaluation of this promisingcombination therapy for the treatment of cancer, including breast andprostate cancer and their bone metastases.

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The invention claimed is:
 1. A pharmaceutical combination comprising:component A, which is2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide,or a physiologically acceptable salt, solvate, hydrate or stereoisomerthereof; and component B, which is radium-223 dichloride.
 2. Thepharmaceutical combination according to claim 1, wherein component A is2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide,or a physiologically acceptable salt thereof.
 3. The pharmaceuticalcombination according to claim 1, wherein component A is2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamidedihydrochloride.
 4. The pharmaceutical combination according to claim 1,wherein component A is2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamide.5. A method for treatment of hepatocyte carcinoma, lung cancer,non-small cell lung carcinoma, colorectal cancer, melanoma, pancreaticcancer, prostate cancer, breast cancer or metastases thereof, comprisingadministering to a patient in need thereof a therapeutically effectiveamount of the pharmaceutical combination according to claim
 1. 6. A kitcomprising the pharmaceutical combination according to claim 1, andoptionally one or more further pharmaceutical agents C, whereinoptionally both or either of components A and B are in the form of apharmaceutical formulation which is ready for use to be administeredsimultaneously, concurrently, separately or sequentially.
 7. Apharmaceutical composition comprising the pharmaceutical combinationaccording to claim 1 and a pharmaceutically acceptable ingredient.
 8. Amethod for treatment of multiple myeloma, lung cancer, breast cancer,prostate cancer, or metastases thereof, comprising administering to apatient in need thereof a therapeutically effective amount of thepharmaceutical combination according to claim
 1. 9. The method accordingto claim 5, wherein the metastases are bone metastases.
 10. The methodof claim 8, wherein the prostate cancer is castration-resistant prostatecancer (CRPC).
 11. The method of claim 5, wherein the pharmaceuticalcombination comprises2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamideor a physiologically acceptable salt thereof.
 12. The kit of claim 6,wherein the pharmaceutical combination comprises2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamideor a physiologically acceptable salt thereof.
 13. The pharmaceuticalcomposition of claim 7, wherein the pharmaceutical combination comprises2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamideor a physiologically acceptable salt thereof.
 14. The method of claim 8,wherein the pharmaceutical combination comprises2-amino-N-[7-methoxy-8-(3-morpholin-4-ylpropoxy)-2,3-dihydroimidazo[1,2-c]quinazolin-5-yl]pyrimidine-5-carboxamideor a physiologically acceptable salt thereof.